biostudies-literatureZhao QMEXT | Japan Society for the Promotion of ScienceThe University President’ Discretionary ExpensesNakatomi FoundationToyama Dai-ichi Bank Scholarship FoundationTakeda Science FoundationToyama Prefecture Human Resources Development FoundationTamura Science and Technology FoundationENEURO.0121-22.2022https://www.ebi.ac.uk/biostudies/studies/S-EPMC9159529biostudies-literatureUnknown9(3)The dorsal raphe (DR) nucleus contains many tyrosine hydroxylase (TH)-positive neurons which are regarded as dopaminergic (DA) neurons. These DA neurons in the DR and periaqueductal gray (PAG) region (DA^DR-PAG neurons) are a subgroup of the A10 cluster, which is known to be heterogeneous. This DA population projects to the central nucleus of the amygdala (CeA) and the bed nucleus of the stria terminalis (BNST) and has been reported to modulate various affective behaviors. To characterize, the histochemical features of DA^DR-PAG neurons projecting to the CeA and BNST in mice, the current study combined retrograde labeling with Fluoro-Gold (FG) and histological techniques, focusing on TH, dopamine transporter (DAT), vasoactive intestinal peptide (VIP), and vesicular gluteNeuroHistochemical Characterization of the Dorsal Raphe-Periaqueductal Grey Dopamine Transporter Neurons Projecting to the Extended Amygdala.PMC9159529JP21K0674601JP19H04212JP19K0645917H02223JP21H0258901JP19K16192JP21H02592JP21K07489JP19K0991822K09663JP20H0341001JP18KK0468JP20K09209JP20K2166422K15795JP19K24241Ito TKonno KYamasaki MOhtsuka SYamamoto RHioki HOno MSoko CZhao QKato NWatanabe MHori YFuruyama TfalseHistochemical Characterization of the Dorsal Raphe-Periaqueductal Grey Dopamine Transporter Neurons Projecting to the Extended Amygdala.The dorsal raphe (DR) nucleus contains many tyrosine hydroxylase (TH)-positive neurons which are regarded as dopaminergic (DA) neurons. These DA neurons in the DR and periaqueductal gray (PAG) region (DA^DR-PAG neurons) are a subgroup of the A10 cluster, which is known to be heterogeneous. This DA population projects to the central nucleus of the amygdala (CeA) and the bed nucleus of the stria terminalis (BNST) and has been reported to modulate various affective behaviors. To characterize, the histochemical features of DA^DR-PAG neurons projecting to the CeA and BNST in mice, the current study combined retrograde labeling with Fluoro-Gold (FG) and histological techniques, focusing on TH, dopamine transporter (DAT), vasoactive intestinal peptide (VIP), and vesicular glut2022-01-01T00:00:00Z2022 May-Jun2025-04-06T04:01:53.308Z2025-04-06T04:01:53.308ZS-EPMC91595293558098610.1523/ENEURO.0121-22.2022