biostudies-literatureUnknown13Vargova RGrantová Agentura České RepublikyEuropean Regional Development FundFornicata, a lineage of a broader and ancient anaerobic eukaryotic clade Metamonada, contains diverse taxa that are ideally suited for evolutionary studies addressing various fundamental biological questions, such as the evolutionary trajectory of mitochondrion-related organelles (MROs), the transition between free-living and endobiotic lifestyles, and the derivation of alternative genetic codes. To this end, we conducted detailed microscopic and transcriptome analyses in a poorly documented strain of an anaerobic free-living marine flagellate, PCS, in the so-called CL3 fornicate lineage. Fortuitously, we discovered that the original culture contained two morphologically similar and closely related CL3 representatives, which doubles the taxon representation within this lineage. We obtainedFrontiers in microbiology866459https://www.ebi.ac.uk/biostudies/studies/S-EPMC9161772biostudies-literatureEvidence for an Independent Hydrogenosome-to-Mitosome Transition in the CL3 Lineage of Fornicates.PMC9161772Vargova RSilberman JDZihala DCepicka ISalamonova JHanouskova PElias MfalseEvidence for an Independent Hydrogenosome-to-Mitosome Transition in the CL3 Lineage of Fornicates.Fornicata, a lineage of a broader and ancient anaerobic eukaryotic clade Metamonada, contains diverse taxa that are ideally suited for evolutionary studies addressing various fundamental biological questions, such as the evolutionary trajectory of mitochondrion-related organelles (MROs), the transition between free-living and endobiotic lifestyles, and the derivation of alternative genetic codes. To this end, we conducted detailed microscopic and transcriptome analyses in a poorly documented strain of an anaerobic free-living marine flagellate, PCS, in the so-called CL3 fornicate lineage. Fortuitously, we discovered that the original culture contained two morphologically similar and closely related CL3 representatives, which doubles the taxon representation within this lineage. We obtained2022-01-01T00:00:00Z20222026-04-08T10:11:56.333Z2024-10-16T11:49:36.018ZS-EPMC91617723566389510.3389/fmicb.2022.866459