biostudies-literatureSui SMajor Science and Technology Program of Changzhou Health Commission in 2020Project of Changzhou Medical Innovation Team10617-10628https://www.ebi.ac.uk/biostudies/studies/S-EPMC9161985biostudies-literatureUnknown13(4)Pancreatic cancer (PC) is a life-threatening cancer with increasing incidence in developed countries. Reports indicate that tRNA-derived fragments (tRFs) are possible therapeutic targets and biomarkers for cancer treatment. Nonetheless, the effect of tRF-Leu-AAG on PC is unclear. This study aims to explore the role of tRF-Leu-AAG and upstream frameshift mutant 1 (UPF1) in the development of PC and its potential underlying mechanisms. High-throughput second-generation sequencing techniques were used to detect the expression of tRFs in cancerous and adjacent normal tissues from PC patients. The role of tRF-Leu-AAG proliferation in PC cells was investigated via the Cell Counting Kit-8 (CCK8) assay. The effect of tRF-Leu-AAG on the invasion and migration ability of PC cells was also determinedBioengineeredThe biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.PMC9161985ZD202023CCX201807Sui SCui XWang ZJin LZhu CfalseThe biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.Pancreatic cancer (PC) is a life-threatening cancer with increasing incidence in developed countries. Reports indicate that tRNA-derived fragments (tRFs) are possible therapeutic targets and biomarkers for cancer treatment. Nonetheless, the effect of tRF-Leu-AAG on PC is unclear. This study aims to explore the role of tRF-Leu-AAG and upstream frameshift mutant 1 (UPF1) in the development of PC and its potential underlying mechanisms. High-throughput second-generation sequencing techniques were used to detect the expression of tRFs in cancerous and adjacent normal tissues from PC patients. The role of tRF-Leu-AAG proliferation in PC cells was investigated via the Cell Counting Kit-8 (CCK8) assay. The effect of tRF-Leu-AAG on the invasion and migration ability of PC cells was also determined2022-01-01T00:00:00Z2022 Apr2025-04-22T11:53:33.373Z2025-04-06T00:05:34.16ZS-EPMC91619853544215210.1080/21655979.2022.2064206