{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wiseman AK"],"funding":["American Cancer Society","U.S. Department of Health & Human Services | NIH | National Cancer Institute","NCI NIH HHS","American Cancer Society, Michigan Cancer Research Fund"],"pagination":["528"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9163065"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(1)"],"pubmed_abstract":["The DNA methylation status of the X-chromosome in cancer cells is often overlooked because of computational difficulties. Most of the CpG islands on the X-chromosome are mono-allelically methylated in normal female cells and only present as a single copy in male cells. We treated two colorectal cancer cell lines from a male (HCT116) and a female (RKO) with increasing doses of a DNA methyltransferase 1 (DNMT1)-specific inhibitor (GSK3685032/GSK5032) over several months to remove as much non-essential CpG methylation as possible. Profiling of the remaining DNA methylome revealed an unexpected, enriched retention of DNA methylation on the X-chromosome. Strikingly, the identified retained X-chromosome DNA methylation patterns accurately predicted de novo DNA hypermethylation in colon cancer pa"],"journal":["Communications biology"],"pubmed_title":["Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1."],"pmcid":["PMC9163065"],"funding_grant_id":["R35CA209859","R35 CA209859","PF-16-245-01-DMB"],"pubmed_authors":["Fan H","Tiedemann RL","McCabe MT","Madaj Z","Pappalardi MB","Wiseman AK","Jones PA","Shen H"],"additional_accession":[]},"is_claimable":false,"name":"Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1.","description":"The DNA methylation status of the X-chromosome in cancer cells is often overlooked because of computational difficulties. Most of the CpG islands on the X-chromosome are mono-allelically methylated in normal female cells and only present as a single copy in male cells. We treated two colorectal cancer cell lines from a male (HCT116) and a female (RKO) with increasing doses of a DNA methyltransferase 1 (DNMT1)-specific inhibitor (GSK3685032/GSK5032) over several months to remove as much non-essential CpG methylation as possible. Profiling of the remaining DNA methylome revealed an unexpected, enriched retention of DNA methylation on the X-chromosome. Strikingly, the identified retained X-chromosome DNA methylation patterns accurately predicted de novo DNA hypermethylation in colon cancer pa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-25T19:45:03.892Z","creation":"2025-04-06T08:08:22.935Z"},"accession":"S-EPMC9163065","cross_references":{"pubmed":["35654826"],"doi":["10.1038/s42003-022-03509-3"]}}