{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li W"],"funding":["National Institute of Neurological Disorders and Stroke","NINDS NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e1010013"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9166358"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(5)"],"pubmed_abstract":["Each day and in conjunction with ambient daylight conditions, neuropeptide PDF regulates the phase and amplitude of locomotor activity rhythms in Drosophila through its receptor, PDFR, a Family B G protein-coupled receptor (GPCR). We studied the in vivo process by which PDFR signaling turns off, by converting as many as half of the 28 potential sites of phosphorylation in its C terminal tail to a non-phosphorylatable residue (alanine). We report that many such sites are conserved evolutionarily, and their conversion creates a specific behavioral syndrome opposite to loss-of-function phenotypes previously described for pdfr. That syndrome includes increases in the amplitudes of both Morning and Evening behavioral peaks, as well as multi-hour delays of the Evening phase. The precise behavioral effects were dependent on day-length, and most effects mapped to conversion of only a few, specific serine residues near the very end of the protein and specific to its A isoform. Behavioral phase delays of the Evening activity under entraining conditions predicted the phase of activity cycles under constant darkness. The behavioral phenotypes produced by the most severe PDFR variant were ligand-dependent in vivo, and not a consequence of changes to their pharmacological properties, nor of changes in their surface expression, as measured in vitro. The mechanisms underlying termination of PDFR signaling are complex, subject to regulation that is modified by season, and central to a better understanding of the peptidergic modulation of behavior."],"journal":["PLoS genetics"],"pubmed_title":["Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila."],"pmcid":["PMC9166358"],"funding_grant_id":["R01 NS108393-20","R01 GM127508-04","R01 NS108393","R01 GM127508"],"pubmed_authors":["Li W","Taghert PH","Trigg JS"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila.","description":"Each day and in conjunction with ambient daylight conditions, neuropeptide PDF regulates the phase and amplitude of locomotor activity rhythms in Drosophila through its receptor, PDFR, a Family B G protein-coupled receptor (GPCR). We studied the in vivo process by which PDFR signaling turns off, by converting as many as half of the 28 potential sites of phosphorylation in its C terminal tail to a non-phosphorylatable residue (alanine). We report that many such sites are conserved evolutionarily, and their conversion creates a specific behavioral syndrome opposite to loss-of-function phenotypes previously described for pdfr. That syndrome includes increases in the amplitudes of both Morning and Evening behavioral peaks, as well as multi-hour delays of the Evening phase. The precise behavioral effects were dependent on day-length, and most effects mapped to conversion of only a few, specific serine residues near the very end of the protein and specific to its A isoform. Behavioral phase delays of the Evening activity under entraining conditions predicted the phase of activity cycles under constant darkness. The behavioral phenotypes produced by the most severe PDFR variant were ligand-dependent in vivo, and not a consequence of changes to their pharmacological properties, nor of changes in their surface expression, as measured in vitro. The mechanisms underlying termination of PDFR signaling are complex, subject to regulation that is modified by season, and central to a better understanding of the peptidergic modulation of behavior.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-25T19:47:31.19Z","creation":"2025-04-06T08:08:20.138Z"},"accession":"S-EPMC9166358","cross_references":{"pubmed":["35605015"],"doi":["10.1371/journal.pgen.1010013"]}}