{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun P"],"funding":["Natural Science Foundation of Shandong Province","National Natural Science Foundation of China"],"pagination":["228"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9166479"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["<h4>Background</h4>Corneal transplantation is the only way to treat serious corneal diseases caused by corneal endothelial dysfunction. However, the shortage of donor corneal tissues and human corneal endothelial cells (HCECs) remains a worldwide challenge. We cultivated HCECs by the use of a conditioned medium from orbital adipose-derived stem cells (OASC-CM) in vitro. Then the HCECs were used to treat animal corneal endothelial dysfunction models via cell transplantation. The purpose of this study was to conduct a long-term observation and evaluation after cell transplantation.<h4>Methods</h4>Orbital adipose-derived stem cells (OASCs) were isolated to prepare the conditioned medium (CM). HCECs were cultivated and expanded by the usage of the CM (CM-HCECs). Then, related corneal endotheli"],"journal":["Stem cell research & therapy"],"pubmed_title":["Long-term observation after transplantation of cultured human corneal endothelial cells for corneal endothelial dysfunction."],"pmcid":["PMC9166479"],"funding_grant_id":["81770893","ZR2020QH145"],"pubmed_authors":["Shen L","Li YB","Sun P","Du LQ","Wu XY"],"additional_accession":[]},"is_claimable":false,"name":"Long-term observation after transplantation of cultured human corneal endothelial cells for corneal endothelial dysfunction.","description":"<h4>Background</h4>Corneal transplantation is the only way to treat serious corneal diseases caused by corneal endothelial dysfunction. However, the shortage of donor corneal tissues and human corneal endothelial cells (HCECs) remains a worldwide challenge. We cultivated HCECs by the use of a conditioned medium from orbital adipose-derived stem cells (OASC-CM) in vitro. Then the HCECs were used to treat animal corneal endothelial dysfunction models via cell transplantation. The purpose of this study was to conduct a long-term observation and evaluation after cell transplantation.<h4>Methods</h4>Orbital adipose-derived stem cells (OASCs) were isolated to prepare the conditioned medium (CM). HCECs were cultivated and expanded by the usage of the CM (CM-HCECs). Then, related corneal endotheli","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-21T14:16:07.103Z","creation":"2025-04-21T14:16:07.103Z"},"accession":"S-EPMC9166479","cross_references":{"pubmed":["35659288"],"doi":["10.1186/s13287-022-02889-x"]}}