biostudies-literatureSun PNatural Science Foundation of Shandong ProvinceNational Natural Science Foundation of China228https://www.ebi.ac.uk/biostudies/studies/S-EPMC9166479biostudies-literatureUnknown13(1)<h4>Background</h4>Corneal transplantation is the only way to treat serious corneal diseases caused by corneal endothelial dysfunction. However, the shortage of donor corneal tissues and human corneal endothelial cells (HCECs) remains a worldwide challenge. We cultivated HCECs by the use of a conditioned medium from orbital adipose-derived stem cells (OASC-CM) in vitro. Then the HCECs were used to treat animal corneal endothelial dysfunction models via cell transplantation. The purpose of this study was to conduct a long-term observation and evaluation after cell transplantation.<h4>Methods</h4>Orbital adipose-derived stem cells (OASCs) were isolated to prepare the conditioned medium (CM). HCECs were cultivated and expanded by the usage of the CM (CM-HCECs). Then, related corneal endotheliStem cell research & therapyLong-term observation after transplantation of cultured human corneal endothelial cells for corneal endothelial dysfunction.PMC916647981770893ZR2020QH145Shen LLi YBSun PDu LQWu XYfalseLong-term observation after transplantation of cultured human corneal endothelial cells for corneal endothelial dysfunction.<h4>Background</h4>Corneal transplantation is the only way to treat serious corneal diseases caused by corneal endothelial dysfunction. However, the shortage of donor corneal tissues and human corneal endothelial cells (HCECs) remains a worldwide challenge. We cultivated HCECs by the use of a conditioned medium from orbital adipose-derived stem cells (OASC-CM) in vitro. Then the HCECs were used to treat animal corneal endothelial dysfunction models via cell transplantation. The purpose of this study was to conduct a long-term observation and evaluation after cell transplantation.<h4>Methods</h4>Orbital adipose-derived stem cells (OASCs) were isolated to prepare the conditioned medium (CM). HCECs were cultivated and expanded by the usage of the CM (CM-HCECs). Then, related corneal endotheli2022-01-01T00:00:00Z2022 Jun2025-04-21T14:16:07.103Z2025-04-21T14:16:07.103ZS-EPMC91664793565928810.1186/s13287-022-02889-x