biostudies-literatureYang XNational Natural Science Foundation of China9285https://www.ebi.ac.uk/biostudies/studies/S-EPMC9166803biostudies-literatureUnknown12(1)This research is to investigate the expression of the TGF-β1/Smads/α-SMA pathway and its effect on bladder histology and function in children with neurogenic bladder (NB). The bladder specimens from 10 children with NB and 8 children with vesicoureteral junction obstruction were collected into the NB and control groups. The expression of TGF-β1, Smad2, Smad3, Smad4, Smad6, α-SMA, fibronectin, collagen I and collagen III in bladder tissues was detected. In addition, the histological characteristics of the bladder were evaluated. A preoperative urodynamic study was performed on all children with NB. We analysed the correlations among the expression of the marker protein a-SMA in myofibroblasts, effector cells of the pathway, and bladder function parameters. Compared with those in the controlScientific reportsActivation of the TGF-β1/Smads/α-SMA pathway is related to histological and functional changes in children with neurogenic bladder.PMC9166803U1904208Yang XPu QWen YLv LXu PMa YWen JGWang JLiu EZhao YfalseActivation of the TGF-β1/Smads/α-SMA pathway is related to histological and functional changes in children with neurogenic bladder.This research is to investigate the expression of the TGF-β1/Smads/α-SMA pathway and its effect on bladder histology and function in children with neurogenic bladder (NB). The bladder specimens from 10 children with NB and 8 children with vesicoureteral junction obstruction were collected into the NB and control groups. The expression of TGF-β1, Smad2, Smad3, Smad4, Smad6, α-SMA, fibronectin, collagen I and collagen III in bladder tissues was detected. In addition, the histological characteristics of the bladder were evaluated. A preoperative urodynamic study was performed on all children with NB. We analysed the correlations among the expression of the marker protein a-SMA in myofibroblasts, effector cells of the pathway, and bladder function parameters. Compared with those in the control2022-01-01T00:00:00Z2022 Jun2025-04-25T19:07:55.92Z2025-04-06T07:50:11.215ZS-EPMC91668033566226810.1038/s41598-022-13470-0