biostudies-literatureVenis SMNCATS NIH HHSNHLBI NIH HHSNCI NIH HHSNational Institutes of Health3675-3685https://www.ebi.ac.uk/biostudies/studies/S-EPMC9175079biostudies-literatureUnknown21(19)A pancreatic acinus is a functional unit of the exocrine pancreas producing digest enzymes. Its pathobiology is crucial to pancreatic diseases including pancreatitis and pancreatic cancer, which can initiate from pancreatic acini. However, research on pancreatic acini has been significantly hampered due to the difficulty of culturing normal acinar cells <i>in vitro</i>. In this study, an <i>in vitro</i> model of the normal acinus, named pancreatic acinus-on-chip (PAC), is developed using reprogrammed pancreatic cancer cells. The developed model is a microfluidic platform with an epithelial duct and acinar sac geometry microfabricated by a newly developed two-step controlled "viscous-fingering" technique. In this model, human pancreatic cancer cells, Panc-1, reprogrammed to revert to the noLab on a chipEngineering of a functional pancreatic acinus with reprogrammed cancer cells by induced <i>PTF1a</i> expression.PMC9175079R01 CA124586R01 CA254110P30 CA023168P30 CA082709R01 CA211098UL1 TR002529U01 HL143403Moon HRKonieczny SFYang YHan BVenis SMUtturkar SMfalseEngineering of a functional pancreatic acinus with reprogrammed cancer cells by induced <i>PTF1a</i> expression.A pancreatic acinus is a functional unit of the exocrine pancreas producing digest enzymes. Its pathobiology is crucial to pancreatic diseases including pancreatitis and pancreatic cancer, which can initiate from pancreatic acini. However, research on pancreatic acini has been significantly hampered due to the difficulty of culturing normal acinar cells <i>in vitro</i>. In this study, an <i>in vitro</i> model of the normal acinus, named pancreatic acinus-on-chip (PAC), is developed using reprogrammed pancreatic cancer cells. The developed model is a microfluidic platform with an epithelial duct and acinar sac geometry microfabricated by a newly developed two-step controlled "viscous-fingering" technique. In this model, human pancreatic cancer cells, Panc-1, reprogrammed to revert to the no2021-01-01T00:00:00Z2021 Sep2026-05-09T20:13:16.736Z2024-10-19T06:48:59.171ZS-EPMC91750793458171910.1039/d1lc00350j