biostudies-literatureZhang YMike Slive Foundation for Prostate Cancer ResearchU.S. Department of Health & Human Services | NIH | National Cancer InstituteU.S. Department of DefenseNCI NIH HHS3186-3196https://www.ebi.ac.uk/biostudies/studies/S-EPMC9177620biostudies-literatureUnknown41(23)For human prostate cancer, the chromosome 8p21 locus, which contains NKX3.1 and the microRNA (miR)-3622 family (miR-3622a/b), is a frequently deleted region. Thus, miR-3622 is proposed as a suppressor for prostate cancer, but its role remains debatable. In the present study, we found that expression of miR-3622a was lower, whereas expression of miR-3622b-3p was higher in human prostate cancer tissues than in normal prostate tissues. miR-3622a-3p inhibited cell migration and invasion of human prostate cancer cells, whereas miR-3622b-3p facilitated cell proliferation, migration, and invasion. To address the opposing roles of miR-3622 family members in various human prostate cancer cell lines, we knocked out (KO) endogenous miR-3622, including both miR-3622a/b. Our results showed that miR-362OncogeneThe microRNA-3622 family at the 8p21 locus exerts oncogenic effects by regulating the p53-downstream gene network in prostate cancer progression.PMC9177620W81XWH-21-1-0100U54 CA118623U54 CA118948U54 CA118638P30 CA013148W81XWH-20-1-0426CA118948Hu FLiu ZZhang YBae SWei SLiu RZhang CXu ZWen WLi MZhou JWang LfalseThe microRNA-3622 family at the 8p21 locus exerts oncogenic effects by regulating the p53-downstream gene network in prostate cancer progression.For human prostate cancer, the chromosome 8p21 locus, which contains NKX3.1 and the microRNA (miR)-3622 family (miR-3622a/b), is a frequently deleted region. Thus, miR-3622 is proposed as a suppressor for prostate cancer, but its role remains debatable. In the present study, we found that expression of miR-3622a was lower, whereas expression of miR-3622b-3p was higher in human prostate cancer tissues than in normal prostate tissues. miR-3622a-3p inhibited cell migration and invasion of human prostate cancer cells, whereas miR-3622b-3p facilitated cell proliferation, migration, and invasion. To address the opposing roles of miR-3622 family members in various human prostate cancer cell lines, we knocked out (KO) endogenous miR-3622, including both miR-3622a/b. Our results showed that miR-3622022-01-01T00:00:00Z2022 Jun2025-04-19T22:46:31.713Z2025-04-19T22:46:31.713ZS-EPMC91776203550146410.1038/s41388-022-02289-8