biostudies-literatureRajagopalan KSNIDDK NIH HHSNHLBI NIH HHSRegenerative Medicine MinnesotaNIH1803https://www.ebi.ac.uk/biostudies/studies/S-EPMC9180447biostudies-literatureUnknown11(11)<h4>Background</h4>Scattered tubular-like cells (STCs) are dedifferentiated renal tubular cells endowed with progenitor-like characteristics to repair injured parenchymal cells. STCs may be damaged and rendered ineffective by renal artery stenosis (RAS), but the underlying processes remain unclear. We hypothesized that RAS alters the epigenetic landscape on DNA and the ensuing gene transcriptional profile of swine STCs.<h4>Methods</h4>CD24+/CD133+ STCs were isolated from pig kidneys after 10 weeks of RAS or sham (<i>n</i> = 3 each) and their whole 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) profiles were examined by 5mC and 5hmC immunoprecipitation sequencing (MeDIP-/hMeDIP-seq, respectively). A subsequent integrated (MeDIP/hMeDIP-seq/mRNA-seq) analysis was performed by compaCellsRenal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells.PMC9180447HL095638DK129240RMM 091620 DS 004R01 DK122734R01 DK129240R56 DK129240K08 DK118120R21 DK118391DK118120R01 DK128017DK122734R01 HL095638DK118391Herrmann SMGlasstetter LMJordan KLIrazabal MVSaadiq IMLerman LOEirin AChade ARZhu XYThaler RRajagopalan KSTang HfalseRenal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells.<h4>Background</h4>Scattered tubular-like cells (STCs) are dedifferentiated renal tubular cells endowed with progenitor-like characteristics to repair injured parenchymal cells. STCs may be damaged and rendered ineffective by renal artery stenosis (RAS), but the underlying processes remain unclear. We hypothesized that RAS alters the epigenetic landscape on DNA and the ensuing gene transcriptional profile of swine STCs.<h4>Methods</h4>CD24+/CD133+ STCs were isolated from pig kidneys after 10 weeks of RAS or sham (<i>n</i> = 3 each) and their whole 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) profiles were examined by 5mC and 5hmC immunoprecipitation sequencing (MeDIP-/hMeDIP-seq, respectively). A subsequent integrated (MeDIP/hMeDIP-seq/mRNA-seq) analysis was performed by compa2022-01-01T00:00:00Z2022 May2026-04-08T10:02:22.452Z2025-04-05T19:55:53.313ZS-EPMC91804473568149810.3390/cells11111803