biostudies-literatureSun Mthe National Key Research and Development Program of China-Technology Integration and demonstration for improving the quality and effectiveness of tea in Dabie Mountainthe Key Science and Technology Research Project of Henan Provincethe Key Scientific Research Project of Higher Education Institutions in Henan province3562https://www.ebi.ac.uk/biostudies/studies/S-EPMC9182030biostudies-literatureUnknown27(11)As important biomolecules in <i>Camellia sinensis</i> L., amino acids (AAs) are considered to contribute to the overall green tea sensory quality and undergo dynamic changes during growth. However, limited by analytical capacity, detailed AAs composition in different growth stages remains unclear. To address this question, we analyzed the dynamic changes of 23 AAs during leaf growth in Xinyang Mao Jian (XYMJ) green tea. Using amino acid analyzer, we demonstrated that most AAs are abundant on Pure Brightness Day and Grain Rain Day. After Grain Rain, 23 AAs decreased significantly. Further analysis shows that theanine has a high level on the day before Spring Equinox and Grain Rain, accounting for 44-61% of the total free AAs content in tea leaves. Glu, Pro, and Asp are the second most abundMolecules (Basel, Switzerland)Dynamic Variation of Amino Acid Contents and Identification of Sterols in Xinyang Mao Jian Green Tea.PMC91820302021YFD160110321210231110921A430032Zhang WSun MChen MTian YJiang SYang RYan YYuan HYang FHou WfalseDynamic Variation of Amino Acid Contents and Identification of Sterols in Xinyang Mao Jian Green Tea.As important biomolecules in <i>Camellia sinensis</i> L., amino acids (AAs) are considered to contribute to the overall green tea sensory quality and undergo dynamic changes during growth. However, limited by analytical capacity, detailed AAs composition in different growth stages remains unclear. To address this question, we analyzed the dynamic changes of 23 AAs during leaf growth in Xinyang Mao Jian (XYMJ) green tea. Using amino acid analyzer, we demonstrated that most AAs are abundant on Pure Brightness Day and Grain Rain Day. After Grain Rain, 23 AAs decreased significantly. Further analysis shows that theanine has a high level on the day before Spring Equinox and Grain Rain, accounting for 44-61% of the total free AAs content in tea leaves. Glu, Pro, and Asp are the second most abund2022-01-01T00:00:00Z2022 Jun2025-04-22T00:26:05.75Z2024-11-08T13:22:56.789ZS-EPMC91820303568449910.3390/molecules27113562