biostudies-literatureWang CZhejiang A &amp; F University1491https://www.ebi.ac.uk/biostudies/studies/S-EPMC9182992biostudies-literatureUnknown11(11)In the process of growth and development, cotton exhibits premature senescence under various abiotic stresses, impairing yield and fiber quality. NAC (NAM, ATAF1,2, and CUC2) protein widely distributed in land plants, play the critical role in responding to abiotic stress and regulating leaf senescence. We have identified and functional analyzed a NAM domain gene <i>GhNAC82</i> in upland cotton, it was located on the A11 chromosome 4,921,702 to 4,922,748 bp, only containing one exon. The spatio-temporal expression pattern analysis revealed that it was highly expressed in root, torus, ovule and fiber development stage. The results of qRT-PCR validated that <i>GhNAC82</i> negatively regulated by salt stress, drought stress, H₂O₂ stress, IAA treatment, and ethylene treatment, positively regulated by the ABA and MeJA treatment. Moreover, heterologous overexpression of <i>GhNAC82</i> results in leaf premature senescence and delays root system development in <i>Arabidopsis thaliana</i>. The phenotype of delayed-senescence was performed after silencing <i>GhNAC82</i> by VIGS in premature cotton. Taken together, <i>GhNAC82</i> was involved in different abiotic stress pathways and play important roles in negatively regulating leaf premature senescence.Plants (Basel, Switzerland)Characterization and Functional Analysis of <i>GhNAC82</i>, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (<i>Gossypium hirsutum</i> L.).PMC91829922021LFR005Feng ZLi TYu SWang CLiu QLi LfalseCharacterization and Functional Analysis of <i>GhNAC82</i>, A NAM Domain Gene, Coordinates the Leaf Senescence in Upland Cotton (<i>Gossypium hirsutum</i> L.).In the process of growth and development, cotton exhibits premature senescence under various abiotic stresses, impairing yield and fiber quality. NAC (NAM, ATAF1,2, and CUC2) protein widely distributed in land plants, play the critical role in responding to abiotic stress and regulating leaf senescence. We have identified and functional analyzed a NAM domain gene <i>GhNAC82</i> in upland cotton, it was located on the A11 chromosome 4,921,702 to 4,922,748 bp, only containing one exon. The spatio-temporal expression pattern analysis revealed that it was highly expressed in root, torus, ovule and fiber development stage. The results of qRT-PCR validated that <i>GhNAC82</i> negatively regulated by salt stress, drought stress, H₂O₂ stress, IAA treatment, and ethylene treatment, positively regulated by the ABA and MeJA treatment. Moreover, heterologous overexpression of <i>GhNAC82</i> results in leaf premature senescence and delays root system development in <i>Arabidopsis thaliana</i>. The phenotype of delayed-senescence was performed after silencing <i>GhNAC82</i> by VIGS in premature cotton. Taken together, <i>GhNAC82</i> was involved in different abiotic stress pathways and play important roles in negatively regulating leaf premature senescence.2022-01-01T00:00:00Z2022 Jun2024-11-10T08:45:11.54Z2024-11-10T08:45:11.54ZS-EPMC91829923568426410.3390/plants11111491