biostudies-literatureUnknown169(1)Calhoun MCElectrochemical sensors that utilize enzymes are a sensitive, inexpensive means of detecting biologically relevant analytes. These sensors are categorized based on their construction and method of signal transport. Type I sensors consist of a crosslinked enzyme on an electrode surface and are potentially subject to interference from byproducts and other biological analytes. However, type II sensors help alleviate this problem with the addition of a redox polymer layer that assists in signal transduction, thus minimizing interferences. An osmium-loaded poly(vinylimidazole) polymer (Os-PVI) is commonly used with successful results, and when combined with an enzyme yields a type II sensor. Our initial attempts at the synthesis of this polymer resulted in an unexpected osmium precursor, which Journal of the Electrochemical Society016506https://www.ebi.ac.uk/biostudies/studies/S-EPMC9183174biostudies-literatureTrace Oxygen Affects Osmium Redox Polymer Synthesis for Wired Enzymatic Biosensors.PMC9183174Schley NDCalhoun MCStachurski CDCliffel DEWinn SLGizzie EADaniel AWfalseTrace Oxygen Affects Osmium Redox Polymer Synthesis for Wired Enzymatic Biosensors.Electrochemical sensors that utilize enzymes are a sensitive, inexpensive means of detecting biologically relevant analytes. These sensors are categorized based on their construction and method of signal transport. Type I sensors consist of a crosslinked enzyme on an electrode surface and are potentially subject to interference from byproducts and other biological analytes. However, type II sensors help alleviate this problem with the addition of a redox polymer layer that assists in signal transduction, thus minimizing interferences. An osmium-loaded poly(vinylimidazole) polymer (Os-PVI) is commonly used with successful results, and when combined with an enzyme yields a type II sensor. Our initial attempts at the synthesis of this polymer resulted in an unexpected osmium precursor, which 2022-01-01T00:00:00Z2022 Jan2025-04-25T20:28:17.562Z2025-02-19T03:52:16.924ZS-EPMC91831743569237010.1149/1945-7111/ac42a0