{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(10)"],"submitter":["Shen HF"],"pubmed_abstract":["To master the technology of reprogramming mouse somatic cells to induced pluripotent stem cells (iPSCs), which will lay a good foundation for setting up a technology platform on reprogramming human cancer cells into iPSCs. Mouse iPSCs (i.e., Oct4-GFP miPSCs) was successfully generated from mouse embryonic fibroblasts (MEFs) harboring Oct4-EGFP transgene by introducing four factors, Oct4, Sox2, c-Myc and Klf4, under mESC (Murine embryonic stem cells) culture conditions. Oct4-GFP miPSCs were similar to mESCs in morphology, proliferation, mESC-specific surface antigens and gene expression. Additionally, Oct4-GFP miPSCs could be cultured in suspension to form embryoid bodies (EBs) and differentiate into cell types of the three germ layers <i>in vitro</i>. Moreover, Oct4-GFP miPSCs could develo"],"journal":["Aging"],"pagination":["4445-4458"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9186763"],"repository":["biostudies-literature"],"pubmed_title":["A real-time pluripotency reporter for the long-term and real-time monitoring of pluripotency changes in induced pluripotent stem cells."],"pmcid":["PMC9186763"],"pubmed_authors":["Xia JW","Li YL","Lin XL","Yao ZF","Zhou Y","Sun Y","Tian YG","Li YC","Zhao WT","Shi JW","Huang SH","Xiao GF","Xiao D","Jia JS","Shen HF"],"additional_accession":[]},"is_claimable":false,"name":"A real-time pluripotency reporter for the long-term and real-time monitoring of pluripotency changes in induced pluripotent stem cells.","description":"To master the technology of reprogramming mouse somatic cells to induced pluripotent stem cells (iPSCs), which will lay a good foundation for setting up a technology platform on reprogramming human cancer cells into iPSCs. Mouse iPSCs (i.e., Oct4-GFP miPSCs) was successfully generated from mouse embryonic fibroblasts (MEFs) harboring Oct4-EGFP transgene by introducing four factors, Oct4, Sox2, c-Myc and Klf4, under mESC (Murine embryonic stem cells) culture conditions. Oct4-GFP miPSCs were similar to mESCs in morphology, proliferation, mESC-specific surface antigens and gene expression. Additionally, Oct4-GFP miPSCs could be cultured in suspension to form embryoid bodies (EBs) and differentiate into cell types of the three germ layers <i>in vitro</i>. Moreover, Oct4-GFP miPSCs could develo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-04T08:46:43.326Z","creation":"2025-04-04T08:46:43.326Z"},"accession":"S-EPMC9186763","cross_references":{"pubmed":["35575836"],"doi":["10.18632/aging.204083"]}}