{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2022"],"submitter":["Yang Y"],"pubmed_abstract":["Mitochondria are versatile organelles and function by communicating with cellular ecosystems. The fluorescent colocalization analysis after fixation is a highly intuitive method to understand the role of mitochondria. However, there are few fluorescent dyes available for mitochondrial staining after fixation. In this study, a novel fluorescent dye (BO-dye), extracted from <i>Buddleja officinalis</i>, was applied for mitochondrial staining in fixed immortalized human oral keratinocytes. The BO-dye (excitation: 414 nm, emission: 677 nm) is a small fluorescent molecular dye, which can cross the cytomembrane without permeabilization. We assume that the BO-dye could aggregate and bind to the mitochondria stably. BO-dye exhibited a mega-Stokes shift (>250 nm), which is an important feature that "],"journal":["Scanning"],"pagination":["7486005"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9187463"],"repository":["biostudies-literature"],"pubmed_title":["A Novel Fluorescent Dye Extracted from <i>Buddleja officinalis</i> for Labeling Mitochondria after Fixation."],"pmcid":["PMC9187463"],"pubmed_authors":["Kim OS","Yang Y","Kim B","Kim Y","Liu D","Kim O","Song J","Liu G","Ma G"],"additional_accession":[]},"is_claimable":false,"name":"A Novel Fluorescent Dye Extracted from <i>Buddleja officinalis</i> for Labeling Mitochondria after Fixation.","description":"Mitochondria are versatile organelles and function by communicating with cellular ecosystems. The fluorescent colocalization analysis after fixation is a highly intuitive method to understand the role of mitochondria. However, there are few fluorescent dyes available for mitochondrial staining after fixation. In this study, a novel fluorescent dye (BO-dye), extracted from <i>Buddleja officinalis</i>, was applied for mitochondrial staining in fixed immortalized human oral keratinocytes. The BO-dye (excitation: 414 nm, emission: 677 nm) is a small fluorescent molecular dye, which can cross the cytomembrane without permeabilization. We assume that the BO-dye could aggregate and bind to the mitochondria stably. BO-dye exhibited a mega-Stokes shift (>250 nm), which is an important feature that ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-21T14:15:27.117Z","creation":"2024-11-09T12:33:39.307Z"},"accession":"S-EPMC9187463","cross_references":{"pubmed":["35711296"],"doi":["10.1155/2022/7486005"]}}