biostudies-literatureUnknown2022Yang YMitochondria are versatile organelles and function by communicating with cellular ecosystems. The fluorescent colocalization analysis after fixation is a highly intuitive method to understand the role of mitochondria. However, there are few fluorescent dyes available for mitochondrial staining after fixation. In this study, a novel fluorescent dye (BO-dye), extracted from <i>Buddleja officinalis</i>, was applied for mitochondrial staining in fixed immortalized human oral keratinocytes. The BO-dye (excitation: 414 nm, emission: 677 nm) is a small fluorescent molecular dye, which can cross the cytomembrane without permeabilization. We assume that the BO-dye could aggregate and bind to the mitochondria stably. BO-dye exhibited a mega-Stokes shift (>250 nm), which is an important feature that Scanning7486005https://www.ebi.ac.uk/biostudies/studies/S-EPMC9187463biostudies-literatureA Novel Fluorescent Dye Extracted from <i>Buddleja officinalis</i> for Labeling Mitochondria after Fixation.PMC9187463Kim OSYang YKim BKim YLiu DKim OSong JLiu GMa GfalseA Novel Fluorescent Dye Extracted from <i>Buddleja officinalis</i> for Labeling Mitochondria after Fixation.Mitochondria are versatile organelles and function by communicating with cellular ecosystems. The fluorescent colocalization analysis after fixation is a highly intuitive method to understand the role of mitochondria. However, there are few fluorescent dyes available for mitochondrial staining after fixation. In this study, a novel fluorescent dye (BO-dye), extracted from <i>Buddleja officinalis</i>, was applied for mitochondrial staining in fixed immortalized human oral keratinocytes. The BO-dye (excitation: 414 nm, emission: 677 nm) is a small fluorescent molecular dye, which can cross the cytomembrane without permeabilization. We assume that the BO-dye could aggregate and bind to the mitochondria stably. BO-dye exhibited a mega-Stokes shift (>250 nm), which is an important feature that 2022-01-01T00:00:00Z20222025-04-21T14:15:27.117Z2024-11-09T12:33:39.307ZS-EPMC91874633571129610.1155/2022/7486005