{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cruz Villarreal J"],"funding":["NIA NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["3945-3958"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9188328"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["414(13)"],"pubmed_abstract":["Understanding cell-to-cell variation at the molecular level provides relevant information about biological phenomena and is critical for clinical and biological research. Proteins carry important information not available from single-cell genomics and transcriptomics studies; however, due to the minute amount of proteins in single cells and the complexity of the proteome, quantitative protein analysis at the single-cell level remains challenging. Here, we report an integrated microfluidic platform in tandem with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the detection and quantification of targeted proteins from small cell ensembles (> 10 cells). All necessary steps for the assay are integrated on-chip including cell lysis, protein immun"],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["MIMAS: microfluidic platform in tandem with MALDI mass spectrometry for protein quantification from small cell ensembles."],"pmcid":["PMC9188328"],"funding_grant_id":["R21 AG067488","R21AG067488"],"pubmed_authors":["Ros A","Sandrin TR","Egatz-Gomez A","Coleman PD","Cruz Villarreal J","Kruithoff R","Ros R"],"additional_accession":[]},"is_claimable":false,"name":"MIMAS: microfluidic platform in tandem with MALDI mass spectrometry for protein quantification from small cell ensembles.","description":"Understanding cell-to-cell variation at the molecular level provides relevant information about biological phenomena and is critical for clinical and biological research. Proteins carry important information not available from single-cell genomics and transcriptomics studies; however, due to the minute amount of proteins in single cells and the complexity of the proteome, quantitative protein analysis at the single-cell level remains challenging. Here, we report an integrated microfluidic platform in tandem with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the detection and quantification of targeted proteins from small cell ensembles (> 10 cells). All necessary steps for the assay are integrated on-chip including cell lysis, protein immun","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-28T07:09:51.244Z","creation":"2025-04-06T19:17:08.021Z"},"accession":"S-EPMC9188328","cross_references":{"pubmed":["35385983"],"doi":["10.1007/s00216-022-04038-y"]}}