biostudies-literatureAguilar-Bravo BH2020 research and innovation programmeFondo Europeo de Desarrollo Regional (FEDER)National Institute on Alcohol Abuse and AlcoholismPhD4MDNIAAA NIH HHSUniversity of BarcelonaAgencia de Gestió d'Ajuts Universitaris i de Recerca (AGAUR)Institució Catalana de Recerca i Estudis Avançats (ICREA)Centro de Investigación en Red Enfermedades Hepáticas y Digestivas (CIBERehd)Instituto de Salud Carlos IIIMINECO, AGAUREuropean Foundation for Alcohol Research (ERAB)Ramón y Cajal programUnión EuropeaFondo de Investigación Sanitaria Carlos III (FIS)2180-2195https://www.ebi.ac.uk/biostudies/studies/S-EPMC9189898biostudies-literatureUnknown69(5)Chronic liver diseases are characterized by the expansion of ductular reaction (DR) cells and the expression of liver progenitor cell (LPC) markers. In alcoholic hepatitis (AH), the degree of DR expansion correlates with disease progression and short-term survival. However, little is known about the biological properties of DR cells, their impact on the pathogenesis of human liver disease, and their contribution to tissue repair. In this study, we have evaluated the transcriptomic profile of DR cells by laser capture microdissection in patients with AH and assessed its association with disease progression. The transcriptome analysis of cytokeratin 7-positive (KRT7⁺ ) DR cells uncovered intrinsic gene pathways expressed in DR and genes associated with alcoholic liver disease progHepatology (Baltimore, Md.)Ductular Reaction Cells Display an Inflammatory Profile and Recruit Neutrophils in Alcoholic Hepatitis.PMC91898981U01AA0208216685961U01AA021908PI13/02340U01 AA021908PI12/012651U01AA021908-01-33490PI12/003302014 SGR 708RYC-2010-07249PI17/00673U01 AA026972EA1653IP13/02340CP11/00071SGR17-490FI16/00203Starkel PAguilar-Bravo BPose EPerea LBataller RVallverdu JSancho-Bru PRubio-Tomas TGraupera IArmengol CMathurin PCaballeria JGines PJose Lozano JDubuquoy LArino SBlaya DRodrigo-Torres DColl MLo Nigro AfalseDuctular Reaction Cells Display an Inflammatory Profile and Recruit Neutrophils in Alcoholic Hepatitis.Chronic liver diseases are characterized by the expansion of ductular reaction (DR) cells and the expression of liver progenitor cell (LPC) markers. In alcoholic hepatitis (AH), the degree of DR expansion correlates with disease progression and short-term survival. However, little is known about the biological properties of DR cells, their impact on the pathogenesis of human liver disease, and their contribution to tissue repair. In this study, we have evaluated the transcriptomic profile of DR cells by laser capture microdissection in patients with AH and assessed its association with disease progression. The transcriptome analysis of cytokeratin 7-positive (KRT7⁺ ) DR cells uncovered intrinsic gene pathways expressed in DR and genes associated with alcoholic liver disease prog2019-01-01T00:00:00Z2019 May2025-04-05T20:40:43.217Z2025-04-05T20:40:43.217ZS-EPMC91898983056527110.1002/hep.30472