{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Baksh SS"],"funding":["NHLBI NIH HHS","National Institutes of Health","National Heart Lung and Blood Institute"],"pagination":["102053"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9190063"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["298(6)"],"pubmed_abstract":["miRNA-based cellular fate reprogramming offers an opportunity to investigate the mechanisms of long-term gene silencing. To further understand how genes are silenced in a tissue-specific manner, we leveraged our miRNA-based method of reprogramming fibroblasts into cardiomyocytes. Through screening approaches, we identified three proteins that were downregulated during reprogramming of fibroblasts into cardiomyocytes: heterochromatin protein Cbx1, transcriptional activator protein PurB, and transcription factor Sp3. We show that knockdown of Cbx1, PurB, and Sp3 was sufficient to induce cardiomyocyte gene expression in fibroblasts. Similarly, gene editing to ablate Cbx1, PurB, and Sp3 expression induced fibroblasts to convert into cardiomyocytes in vivo. Furthermore, high-throughput DNA sequ"],"journal":["The Journal of biological chemistry"],"pubmed_title":["A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes."],"pmcid":["PMC9190063"],"funding_grant_id":["R01 HL131814-01A1","R01 HL131814"],"pubmed_authors":["Gomez J","Hodgkinson CP","Pratt RE","Dzau VJ","Baksh SS"],"additional_accession":[]},"is_claimable":false,"name":"A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes.","description":"miRNA-based cellular fate reprogramming offers an opportunity to investigate the mechanisms of long-term gene silencing. To further understand how genes are silenced in a tissue-specific manner, we leveraged our miRNA-based method of reprogramming fibroblasts into cardiomyocytes. Through screening approaches, we identified three proteins that were downregulated during reprogramming of fibroblasts into cardiomyocytes: heterochromatin protein Cbx1, transcriptional activator protein PurB, and transcription factor Sp3. We show that knockdown of Cbx1, PurB, and Sp3 was sufficient to induce cardiomyocyte gene expression in fibroblasts. Similarly, gene editing to ablate Cbx1, PurB, and Sp3 expression induced fibroblasts to convert into cardiomyocytes in vivo. Furthermore, high-throughput DNA sequ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-27T22:23:20.266Z","creation":"2024-11-12T21:41:17.666Z"},"accession":"S-EPMC9190063","cross_references":{"pubmed":["35605661"],"doi":["10.1016/j.jbc.2022.102053"]}}