{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Abd El-Salam M"],"funding":["Mayo Foundation for Medical Research","The State of Sao Paulo Research Foundation (FAPESP)","TWAS-CNPq: The Academy of Sciences for the Developing World (TWAS), Italy and the National Council for Scientific and Technological Development (CNPq), Brazil","NIDDK NIH HHS","Mayo Clinic O???Brien Urology Research Center"],"pagination":["1609-1621"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9190264"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["61(4)"],"pubmed_abstract":["The plant metabolite 3,4,5-tri-O-galloylquinic acid methyl ester (TGAME, compound 6) was synthesized, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to the surface of Madin-Darby canine kidney cells type I (MDCKI) and crystal growth in a Drosophila melanogaster Malpighian tubule (MT) model were investigated. Membrane, cytosolic, and total annexin A1 (AxA1), α-enolase, and heat shock protein 90 (HSP90) amounts were examined by Western blot analysis after subcellular fractionation, then confirmed by immunofluorescence staining of cultured cells. Pretreatment of MDCKI cells with TGAME for up to 6 h significantly diminished COM crystal binding in a concentration-dependent manner. TGAME significantly inhibited AxA1 surface expression by immunofluorescence microsco"],"journal":["Journal of medicinal chemistry"],"pubmed_title":["The Synthesized Plant Metabolite 3,4,5-Tri-O-Galloylquinic Acid Methyl Ester Inhibits Calcium Oxalate Crystal Growth in a Drosophila Model, Downregulates Renal Cell Surface Annexin A1 Expression, and Decreases Crystal Adhesion to Cells."],"pmcid":["PMC9190264"],"funding_grant_id":["U54 DK100227","2011/13630-7","NIH DK100227","190066/2014-8","R01 DK092408"],"pubmed_authors":["Abd El-Salam M","Lieske J","Donate PM","Bastos JK","Coelho EB","Han JJ","Previdi D","Romero MF"],"additional_accession":[]},"is_claimable":false,"name":"The Synthesized Plant Metabolite 3,4,5-Tri-O-Galloylquinic Acid Methyl Ester Inhibits Calcium Oxalate Crystal Growth in a Drosophila Model, Downregulates Renal Cell Surface Annexin A1 Expression, and Decreases Crystal Adhesion to Cells.","description":"The plant metabolite 3,4,5-tri-O-galloylquinic acid methyl ester (TGAME, compound 6) was synthesized, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to the surface of Madin-Darby canine kidney cells type I (MDCKI) and crystal growth in a Drosophila melanogaster Malpighian tubule (MT) model were investigated. Membrane, cytosolic, and total annexin A1 (AxA1), α-enolase, and heat shock protein 90 (HSP90) amounts were examined by Western blot analysis after subcellular fractionation, then confirmed by immunofluorescence staining of cultured cells. Pretreatment of MDCKI cells with TGAME for up to 6 h significantly diminished COM crystal binding in a concentration-dependent manner. TGAME significantly inhibited AxA1 surface expression by immunofluorescence microsco","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Feb","modification":"2025-05-18T12:45:58.251Z","creation":"2025-05-18T12:45:58.251Z"},"accession":"S-EPMC9190264","cross_references":{"pubmed":["29406740"],"doi":["10.1021/acs.jmedchem.7b01566"]}}