{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["Guangzhou Municipal Science and Technology Project","National Natural Science Foundation of China"],"pagination":["104479"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9192802"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(6)"],"pubmed_abstract":["Huanglongbing (<i>HLB</i>) is a devastating disease of citrus, which is mostly caused by <i>Candidatus</i> Liberibacter asiaticus (C<i>Las</i>). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (<i>Catharanthus roseus</i>) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 (<i>Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1</i>)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA <sub"],"journal":["iScience"],"pubmed_title":["Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research."],"pmcid":["PMC9192802"],"funding_grant_id":["201805010002","31570256"],"pubmed_authors":["Wang M","Zhang J","Zhou C","Xu D","Chen W","Lei W","Meng Y","Zhang B","Yuan J","Meng X"],"additional_accession":[]},"is_claimable":false,"name":"Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research.","description":"Huanglongbing (<i>HLB</i>) is a devastating disease of citrus, which is mostly caused by <i>Candidatus</i> Liberibacter asiaticus (C<i>Las</i>). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (<i>Catharanthus roseus</i>) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 (<i>Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1</i>)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA <sub","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-22T06:00:34.382Z","creation":"2024-11-12T10:56:56.024Z"},"accession":"S-EPMC9192802","cross_references":{"pubmed":["35712078"],"doi":["10.1016/j.isci.2022.104479"]}}