biostudies-literatureZhang JGuangzhou Municipal Science and Technology ProjectNational Natural Science Foundation of China104479https://www.ebi.ac.uk/biostudies/studies/S-EPMC9192802biostudies-literatureUnknown25(6)Huanglongbing (<i>HLB</i>) is a devastating disease of citrus, which is mostly caused by <i>Candidatus</i> Liberibacter asiaticus (C<i>Las</i>). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (<i>Catharanthus roseus</i>) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 (<i>Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1</i>)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA <subiScienceExpression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research.PMC919280220180501000231570256Wang MZhang JZhou CXu DChen WLei WMeng YZhang BYuan JMeng XfalseExpression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research.Huanglongbing (<i>HLB</i>) is a devastating disease of citrus, which is mostly caused by <i>Candidatus</i> Liberibacter asiaticus (C<i>Las</i>). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (<i>Catharanthus roseus</i>) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 (<i>Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1</i>)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA <sub2022-01-01T00:00:00Z2022 Jun2025-04-22T06:00:34.382Z2024-11-12T10:56:56.024ZS-EPMC91928023571207810.1016/j.isci.2022.104479