{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Numata S"],"funding":["National Institutes of Health","NIGMS NIH HHS","NIH HHS"],"pagination":["1206-1217"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9199017"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["106(6)"],"pubmed_abstract":["Glucose is a key substrate for supporting sperm energy production and function. Previous studies have demonstrated that sperm glucose uptake is facilitated by several isoforms of the glucose transporters (GLUT). Here, we report that sperm also expresses the Na+-dependent sodium glucose cotransporter (SGLT). This was first suggested by our observation that genetic deletion of the testis-specific Na,K-ATPase α4, which impairs the sperm plasma membrane Na+ gradient, reduces glucose uptake and ATP production. Immunoblot analysis revealed the presence of an SGLT in sperm, with specific expression of isoform 1 (SGLT-1), but not of isoform 2 (SGLT-2). Immunocytochemistry identified SGLT-1 in the mid- and principal piece of the sperm flagellum. Inhibition of SGLT-1 with the isotype-selective inhib"],"journal":["Biology of reproduction"],"pubmed_title":["The sodium-glucose cotransporter isoform 1 (SGLT-1) is important for sperm energetics, motility, and fertility†."],"pmcid":["PMC9199017"],"funding_grant_id":["P30 GM 103326","R01 HD102623","P30 GM103326","P30 CA168524"],"pubmed_authors":["Numata S","McDermott JP","Sanchez G","Blanco G","Mitra A"],"additional_accession":[]},"is_claimable":false,"name":"The sodium-glucose cotransporter isoform 1 (SGLT-1) is important for sperm energetics, motility, and fertility†.","description":"Glucose is a key substrate for supporting sperm energy production and function. Previous studies have demonstrated that sperm glucose uptake is facilitated by several isoforms of the glucose transporters (GLUT). Here, we report that sperm also expresses the Na+-dependent sodium glucose cotransporter (SGLT). This was first suggested by our observation that genetic deletion of the testis-specific Na,K-ATPase α4, which impairs the sperm plasma membrane Na+ gradient, reduces glucose uptake and ATP production. Immunoblot analysis revealed the presence of an SGLT in sperm, with specific expression of isoform 1 (SGLT-1), but not of isoform 2 (SGLT-2). Immunocytochemistry identified SGLT-1 in the mid- and principal piece of the sperm flagellum. Inhibition of SGLT-1 with the isotype-selective inhib","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-05T11:29:29.525Z","creation":"2025-04-05T11:29:29.525Z"},"accession":"S-EPMC9199017","cross_references":{"pubmed":["35420639"],"doi":["10.1093/biolre/ioac052"]}}