{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fukui K"],"funding":["Japan Society for the Promotion of Science"],"pagination":["855-868"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9199185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(6)"],"pubmed_abstract":["Phosphoribulokinase (PRK), one of the enzymes in the Calvin-Benson cycle, is a well-known target of thioredoxin (Trx), which regulates various enzyme activities by the reduction of disulfide bonds in a light-dependent manner. PRK has two Cys pairs conserved in the N-terminal and C-terminal regions, and the N-terminal one near the active site is thought to be responsible for the regulation. The flexible clamp loop located between the N-terminal two Cys residues has been deemed significant to Trx-mediated regulation. However, cyanobacterial PRK is also subject to Trx-dependent activation despite the lack of this clamp loop. We, therefore, compared Trx-mediated regulation of PRK from the cyanobacterium Anabaena sp. PCC 7120 (A.7120_PRK) and that from the land plant Arabidopsis thaliana (AtPRK"],"journal":["Plant & cell physiology"],"pubmed_title":["The Importance of the C-Terminal Cys Pair of Phosphoribulokinase in Phototrophs in Thioredoxin-Dependent Regulation."],"pmcid":["PMC9199185"],"funding_grant_id":["KAKENHI Grant Number [16H06556] and [21H02502]"],"pubmed_authors":["Yokochi Y","Sekiguchi T","Wakabayashi KI","Mihara S","Fukui K","Yoshida K","Hisabori T"],"additional_accession":[]},"is_claimable":false,"name":"The Importance of the C-Terminal Cys Pair of Phosphoribulokinase in Phototrophs in Thioredoxin-Dependent Regulation.","description":"Phosphoribulokinase (PRK), one of the enzymes in the Calvin-Benson cycle, is a well-known target of thioredoxin (Trx), which regulates various enzyme activities by the reduction of disulfide bonds in a light-dependent manner. PRK has two Cys pairs conserved in the N-terminal and C-terminal regions, and the N-terminal one near the active site is thought to be responsible for the regulation. The flexible clamp loop located between the N-terminal two Cys residues has been deemed significant to Trx-mediated regulation. However, cyanobacterial PRK is also subject to Trx-dependent activation despite the lack of this clamp loop. We, therefore, compared Trx-mediated regulation of PRK from the cyanobacterium Anabaena sp. PCC 7120 (A.7120_PRK) and that from the land plant Arabidopsis thaliana (AtPRK","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-04-08T10:32:04.02Z","creation":"2025-02-19T02:57:26.314Z"},"accession":"S-EPMC9199185","cross_references":{"pubmed":["35413120"],"doi":["10.1093/pcp/pcac050"]}}