{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Fang H"],"pubmed_abstract":["The endoplasmic reticulum (ER) is the main storage site of Zn2+, and Zn2+ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn2+ probe ER-Zn targeting ER stress. The probe displayed a specific Zn2+ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn2+ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn2+, but also the observation of the Zn2+ level change under ER stress, elucidating the different behaviors of Zn2+ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capa"],"journal":["Frontiers in pharmacology"],"pagination":["927609"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9207263"],"repository":["biostudies-literature"],"pubmed_title":["An Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Molecule Reveals Zn2+ Micro-Dynamics During Drug-Induced Organelle Ionic Disorder."],"pmcid":["PMC9207263"],"pubmed_authors":["Geng S","Fang H","Li Y","Guo Z","He W","Yao S","Chen Y"],"additional_accession":[]},"is_claimable":false,"name":"An Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Molecule Reveals Zn2+ Micro-Dynamics During Drug-Induced Organelle Ionic Disorder.","description":"The endoplasmic reticulum (ER) is the main storage site of Zn2+, and Zn2+ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn2+ probe ER-Zn targeting ER stress. The probe displayed a specific Zn2+ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn2+ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn2+, but also the observation of the Zn2+ level change under ER stress, elucidating the different behaviors of Zn2+ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T08:48:00.206Z","creation":"2025-04-04T08:48:00.206Z"},"accession":"S-EPMC9207263","cross_references":{"pubmed":["35734408"],"doi":["10.3389/fphar.2022.927609"]}}