biostudies-literatureUnknown13Fang HThe endoplasmic reticulum (ER) is the main storage site of Zn²⁺, and Zn²⁺ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn²⁺ probe ER-Zn targeting ER stress. The probe displayed a specific Zn²⁺ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn²⁺ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn²⁺, but also the observation of the Zn²⁺ level change under ER stress, elucidating the different behaviors of Zn²⁺ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capaFrontiers in pharmacology927609https://www.ebi.ac.uk/biostudies/studies/S-EPMC9207263biostudies-literatureAn Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Molecule Reveals Zn²⁺ Micro-Dynamics During Drug-Induced Organelle Ionic Disorder.PMC9207263Geng SFang HLi YGuo ZHe WYao SChen YfalseAn Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Molecule Reveals Zn²⁺ Micro-Dynamics During Drug-Induced Organelle Ionic Disorder.The endoplasmic reticulum (ER) is the main storage site of Zn²⁺, and Zn²⁺ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn²⁺ probe ER-Zn targeting ER stress. The probe displayed a specific Zn²⁺ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn²⁺ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn²⁺, but also the observation of the Zn²⁺ level change under ER stress, elucidating the different behaviors of Zn²⁺ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capa2022-01-01T00:00:00Z20222025-04-04T08:48:00.206Z2025-04-04T08:48:00.206ZS-EPMC92072633573440810.3389/fphar.2022.927609