{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramovs V"],"funding":["Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)"],"pagination":["1279-1288"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9213820"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(6)"],"pubmed_abstract":["Human induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via"],"journal":["Stem cell reports"],"pubmed_title":["Characterization of the epidermal-dermal junction in hiPSC-derived skin organoids."],"pmcid":["PMC9213820"],"funding_grant_id":["reNEW LUMC"],"pubmed_authors":["Janssen H","Raymond K","Freund C","Ramovs V","Rachidi W","Chuva de Sousa Lopes SM","Gidrol X","Mummery CL","Fuentes I","Pitaval A"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the epidermal-dermal junction in hiPSC-derived skin organoids.","description":"Human induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-27T20:29:00.834Z","creation":"2025-02-19T02:43:46.766Z"},"accession":"S-EPMC9213820","cross_references":{"pubmed":["35561682"],"doi":["10.1016/j.stemcr.2022.04.008"]}}