biostudies-literatureRamovs VNovo Nordisk Foundation Center for Stem Cell Medicine (reNEW)1279-1288https://www.ebi.ac.uk/biostudies/studies/S-EPMC9213820biostudies-literatureUnknown17(6)Human induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane viaStem cell reportsCharacterization of the epidermal-dermal junction in hiPSC-derived skin organoids.PMC9213820reNEW LUMCJanssen HRaymond KFreund CRamovs VRachidi WChuva de Sousa Lopes SMGidrol XMummery CLFuentes IPitaval AfalseCharacterization of the epidermal-dermal junction in hiPSC-derived skin organoids.Human induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via2022-01-01T00:00:00Z2022 Jun2026-05-27T20:29:00.834Z2025-02-19T02:43:46.766ZS-EPMC92138203556168210.1016/j.stemcr.2022.04.008