{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Soncin F"],"funding":["Eunice Kennedy Shriver National Institute of Child Health and Human Development","NICHD NIH HHS","NCATS NIH HHS","University of California, San Diego","National Institutes of Health","California Institute for Regenerative Medicine","American Society for Reproductive Medicine","NIH HHS","NIGMS NIH HHS","National Institute of Child Health and Human Development","National Science Foundation"],"pagination":["1303-1317"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9214048"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(6)"],"pubmed_abstract":["Trophoblast stem cells (TSCs) have recently been derived from human embryos and early-first-trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a bone morphogetic protein 4 (BMP4)-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSCs) into functional trophoblasts; however, those trophoblasts could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to newly developed TSC medium, to derive bona fide TSCs. We show that these cells resemble placenta- and naive hPSC-derived TSCs, based on their transcriptome as well as their in vitro and in vivo differentiation potential. We"],"journal":["Stem cell reports"],"pubmed_title":["Derivation of functional trophoblast stem cells from primed human pluripotent stem cells."],"pmcid":["PMC9214048"],"funding_grant_id":["TG-MCB140074","T32 GM008806","K99 HD091452","UL1 TR001442","2UL1TR001442-06","R01 HD089537","R00 HD091452","EDUC2-08388","R01 HD096260","R01HD-089537","R01-HD096260","T32 GM007198","S10 OD026929","K99-R00-HD091452","T32GM8806","ACI-1548562"],"pubmed_authors":["Cheung VC","Kallol S","Farah O","Kittle R","Jackson MG","Morey R","Fisch KM","Soncin F","Bui T","Pizzo D","Parast MM","Requena DF","Meads M","Horii M","Chousal J"],"additional_accession":[]},"is_claimable":false,"name":"Derivation of functional trophoblast stem cells from primed human pluripotent stem cells.","description":"Trophoblast stem cells (TSCs) have recently been derived from human embryos and early-first-trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a bone morphogetic protein 4 (BMP4)-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSCs) into functional trophoblasts; however, those trophoblasts could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to newly developed TSC medium, to derive bona fide TSCs. We show that these cells resemble placenta- and naive hPSC-derived TSCs, based on their transcriptome as well as their in vitro and in vivo differentiation potential. We","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-04T03:06:18.188Z","creation":"2025-02-19T05:06:17.931Z"},"accession":"S-EPMC9214048","cross_references":{"pubmed":["35594858"],"doi":["10.1016/j.stemcr.2022.04.013"]}}