biostudies-literatureMagnaye KMNIAID NIH HHSNHLBI NIH HHSHHS | NIH | National Institute of Allergy and Infectious DiseasesHHS | NIH | National Heart, Lung, and Blood InstituteDivision of Intramural Research, National Institute of Allergy and Infectious Diseasese2116467119https://www.ebi.ac.uk/biostudies/studies/S-EPMC9214527biostudies-literatureUnknown119(24)Maternal asthma (MA) is among the most consistent risk factors for asthma in children. Possible mechanisms for this observation are epigenetic modifications in utero that have lasting effects on developmental programs in children of mothers with asthma. To test this hypothesis, we performed differential DNA methylation analyses of 398,186 individual CpG sites in primary bronchial epithelial cells (BECs) from 42 nonasthma controls and 88 asthma cases, including 56 without MA (NMA) and 32 with MA. We used weighted gene coexpression network analysis (WGCNA) of 69 and 554 differentially methylated CpGs (DMCs) that were specific to NMA and MA cases, respectively, compared with controls. WGCNA grouped 66 NMA-DMCs and 203 MA-DMCs into two and five comethylation modules, respectively. The eigenvecProceedings of the National Academy of Sciences of the United States of AmericaDNA methylation signatures in airway cells from adult children of asthmatic mothers reflect subtypes of severe asthma.PMC9214527UM1 AI160040U19 AI095230F31 HL143891Jackson DJOber CClay SMNicodemus-Johnson JNaureckas ETHogarth DKHuffman JMagnaye KMWhite SRNaughton KAGern JEAltman MCfalseDNA methylation signatures in airway cells from adult children of asthmatic mothers reflect subtypes of severe asthma.Maternal asthma (MA) is among the most consistent risk factors for asthma in children. Possible mechanisms for this observation are epigenetic modifications in utero that have lasting effects on developmental programs in children of mothers with asthma. To test this hypothesis, we performed differential DNA methylation analyses of 398,186 individual CpG sites in primary bronchial epithelial cells (BECs) from 42 nonasthma controls and 88 asthma cases, including 56 without MA (NMA) and 32 with MA. We used weighted gene coexpression network analysis (WGCNA) of 69 and 554 differentially methylated CpGs (DMCs) that were specific to NMA and MA cases, respectively, compared with controls. WGCNA grouped 66 NMA-DMCs and 203 MA-DMCs into two and five comethylation modules, respectively. The eigenvec2022-01-01T00:00:00Z2022 Jun2025-04-04T03:06:30.81Z2025-02-19T05:06:24.532ZS-EPMC92145273566686810.1073/pnas.2116467119