{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Emerson FJ"],"funding":["NIA NIH HHS","National Institutes of Health","NIH HHS","National Science Foundation"],"pagination":["e445"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9219583"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(6)"],"pubmed_abstract":["Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high-resolution binding profiles of protein factors or to map histones with specific post-translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in Caenorhabditis elegans, a model system of great importance to genomic research. Cell dissociation techniques, which are required for this approach, "],"journal":["Current protocols"],"pubmed_title":["CUT&RUN for Chromatin Profiling in Caenorhabditis elegans."],"pmcid":["PMC9219583"],"funding_grant_id":["R01 AG024425","DGE‐1650441","R56 AG024425"],"pubmed_authors":["Emerson FJ","Lee SS"],"additional_accession":[]},"is_claimable":false,"name":"CUT&RUN for Chromatin Profiling in Caenorhabditis elegans.","description":"Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high-resolution binding profiles of protein factors or to map histones with specific post-translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in Caenorhabditis elegans, a model system of great importance to genomic research. Cell dissociation techniques, which are required for this approach, ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-05T03:20:59.073Z","creation":"2025-04-19T13:26:33.103Z"},"accession":"S-EPMC9219583","cross_references":{"pubmed":["35714350"],"doi":["10.1002/cpz1.445"]}}