biostudies-literatureEmerson FJNIA NIH HHSNational Institutes of HealthNIH HHSNational Science Foundatione445https://www.ebi.ac.uk/biostudies/studies/S-EPMC9219583biostudies-literatureUnknown2(6)Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high-resolution binding profiles of protein factors or to map histones with specific post-translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in Caenorhabditis elegans, a model system of great importance to genomic research. Cell dissociation techniques, which are required for this approach, Current protocolsCUT&RUN for Chromatin Profiling in Caenorhabditis elegans.PMC9219583R01 AG024425DGE‐1650441R56 AG024425Emerson FJLee SSfalseCUT&RUN for Chromatin Profiling in Caenorhabditis elegans.Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high-resolution binding profiles of protein factors or to map histones with specific post-translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in Caenorhabditis elegans, a model system of great importance to genomic research. Cell dissociation techniques, which are required for this approach, 2022-01-01T00:00:00Z2022 Jun2026-05-05T03:20:59.073Z2025-04-19T13:26:33.103ZS-EPMC92195833571435010.1002/cpz1.445