{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Laveriano-Santos EP"],"funding":["Government of Catalonia","Instituto de Salud Carlos III—ISCIII","SHE Foundation-‘la Caixa’ Foundation","CICYT","Ministry of Science, Innovation, and Universities","Centro Nacional de Investigaciones Cardiovasculares","Ministry of Science, Innovation, and Universities for the Ramon y Cajal contract","“la Caixa” Foundation fellowship","Fondo de Investigación Sanitaria- Instituto de Salud Carlos III (ISCIII) and co-funded by the Eu-ropean Regional Development Fund/European Social Fund "A way to make Europe"/"Investing in your future"","Ministry of Science, Innovation, and Universities for the Ramon y Cajal","Generalitat de Catalunya","Instituto de Salud Carlos III","Ministerio de Ciencia e Innovación","Fondo de Investigación Sanitaria- Instituto de Salud Carlos III (ISCIII) and co-funded by the European Regional Development Fund/European Social Fund “A way to make Europe”/”Investing in your future”","Fundació la Marató de TV3","Caixa Foundation","\"la Caixa\" Foundation fellowship"],"pagination":["1167"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9220091"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(6)"],"pubmed_abstract":["This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra- and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population."],"journal":["Antioxidants (Basel, Switzerland)"],"pubmed_title":["Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents."],"pmcid":["PMC9220091"],"funding_grant_id":["FPU17/00785","RYC-2016-19355","(GC) 2017 SGR 196","PID2020-114022RB-I00","CIBEROBN CB12/03/30020","2017 SGR 196","FPU17/00513","AGL2016-75329-R","LCF/PR/MS19/12220001","PI19/01704","369/C/2016","LCF/PR/CE16/10700001","CEX2020-001041-S","FPU17/00513 and FPU17/00785","AGL2016-75329-R and PID2020-114022RB-I00]","FI-SDUR (EMC/3345/2020)"],"pubmed_authors":["Castro-Baquero S","Martinez-Gomez J","Santos-Beneit G","Lamuela-Raventos RM","Ruiz-Leon AM","Cos-Gandoy A","Marhuenda-Munoz M","Tresserra-Rimbau A","Fernandez-Alvira JM","Vallverdu-Queralt A","Laveriano-Santos EP","Martinez-Huelamo M","Jauregui O","Fernandez-Jimenez R","Miguel M","Estruch R","Arancibia-Riveros C","Bodega P","Miliarakis E"],"additional_accession":[]},"is_claimable":false,"name":"Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents.","description":"This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra- and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-07T03:08:16.437Z","creation":"2025-02-19T02:58:36.67Z"},"accession":"S-EPMC9220091","cross_references":{"pubmed":["35740067"],"doi":["10.3390/antiox11061167"]}}