biostudies-literatureUnknown11(6)Jamshidi EMarche Polytechnic UniversityGrapevine Bois noir (BN) is associated with 'Candidatus Phytoplasma solani'. It has been recorded in vineyards throughout Europe as well as in different countries in Asia, where it now constitutes a threat to Iranian viticulture. BN is strictly dependent on 'Ca. P. solani' strains, wild host plants, and insect vectors. The molecular typing of 'Ca. P. solani', based on the nonribosomal gene tuf and the two hypervariable markers vmp1 and stamp, is valuable for the reconstruction and clarification of the pathways of BN spread. In this study, an RFLP analysis was performed on the vmp1 gene, and a single-nucleotide polymorphism analysis confirmed new vmp types in 'Ca. P. solani'. A stamp gene phylogenetic analysis allowed us Biology835https://www.ebi.ac.uk/biostudies/studies/S-EPMC9220303biostudies-literatureMultilocus Genotyping of 'Candidatus Phytoplasma Solani' Associated with Grapevine Bois Noir in Iran.PMC9220303Murolo SRavari SBJamshidi ESalehi MRomanazzi GfalseMultilocus Genotyping of 'Candidatus Phytoplasma Solani' Associated with Grapevine Bois Noir in Iran.Grapevine Bois noir (BN) is associated with 'Candidatus Phytoplasma solani'. It has been recorded in vineyards throughout Europe as well as in different countries in Asia, where it now constitutes a threat to Iranian viticulture. BN is strictly dependent on 'Ca. P. solani' strains, wild host plants, and insect vectors. The molecular typing of 'Ca. P. solani', based on the nonribosomal gene tuf and the two hypervariable markers vmp1 and stamp, is valuable for the reconstruction and clarification of the pathways of BN spread. In this study, an RFLP analysis was performed on the vmp1 gene, and a single-nucleotide polymorphism analysis confirmed new vmp types in 'Ca. P. solani'. A stamp gene phylogenetic analysis allowed us 2022-01-01T00:00:00Z2022 May2026-05-07T03:08:52.315Z2025-02-19T02:58:50.146ZS-EPMC92203033574135610.3390/biology11060835