biostudies-literatureJorgensen RUnited States Department of Agriculture (USDA)USDA/NIFA, Agricultural and Food Research Initiative Competitive ProgramNational Institute of Food and Agriculture (NIFA)Michigan State University (Department of Food Science and Human Nutrition, Project GREEEN, Ag-Bioresearch)Michigan State UniversityAcademic Achievement Graduate AssistantshipHatch projectsUnited States Department of AgricultureJK Kellogg Endowment for Human Nutrition and HealthNational Institute of Food and Agriculture6505https://www.ebi.ac.uk/biostudies/studies/S-EPMC9224339biostudies-literatureUnknown23(12)Wheat allergies are potentially life-threatening because of the high risk of anaphylaxis. Wheats belong to four genotypes represented in thousands of lines and varieties. Monitoring changes to wheat allergens is critical to prevent inadvertent ntroduction of hyper-allergenic varieties via breeding. However, validated methods for this purpose are unavailable at present. As a proof-of-concept study, we tested the hypothesis that salt-soluble wheat allergens in our mouse model will be identical to those reported for humans. Groups of Balb/cJ mice were rendered allergic to durum wheat salt-soluble protein extract (SSPE). Using blood from allergic mice, a mini hyper-IgE plasma bank was created and used in optimizing an IgE Western blotting (IEWB) to identify IgE binding allergens. The LC-MS/MS International journal of molecular sciencesA Mouse-Based Method to Monitor Wheat Allergens in Novel Wheat Lines and Varieties Created by Crossbreeding: Proof-of-Concept Using Durum and <i>A. tauschii</i> Wheats.PMC9224339MICL02023MICL024862018-67017-27876MICL01699Hatch MICL02023, MICL02486 (Accession Number: 1012322), MICL01699Ng PKWGangur VOlson EGao HRaghunath RJorgensen RfalseA Mouse-Based Method to Monitor Wheat Allergens in Novel Wheat Lines and Varieties Created by Crossbreeding: Proof-of-Concept Using Durum and <i>A. tauschii</i> Wheats.Wheat allergies are potentially life-threatening because of the high risk of anaphylaxis. Wheats belong to four genotypes represented in thousands of lines and varieties. Monitoring changes to wheat allergens is critical to prevent inadvertent ntroduction of hyper-allergenic varieties via breeding. However, validated methods for this purpose are unavailable at present. As a proof-of-concept study, we tested the hypothesis that salt-soluble wheat allergens in our mouse model will be identical to those reported for humans. Groups of Balb/cJ mice were rendered allergic to durum wheat salt-soluble protein extract (SSPE). Using blood from allergic mice, a mini hyper-IgE plasma bank was created and used in optimizing an IgE Western blotting (IEWB) to identify IgE binding allergens. The LC-MS/MS 2022-01-01T00:00:00Z2022 Jun2026-05-09T03:09:17.088Z2024-11-09T21:13:42.713ZS-EPMC92243393574294910.3390/ijms23126505