{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peng H"],"funding":["Zhejiang Provincial R&amp;D Program","National Natural Science Foundation of China","Zhejiang Provincial R&amp;amp;D Program"],"pagination":["558"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9224670"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(6)"],"pubmed_abstract":["FRQ (frequency protein), FRH (FRQ-interacting RNA helicase), and WC1 and WC2 (white collar proteins) are major clock elements that govern the circadian rhythm in <i>Neurospora</i><i>crassa</i>. However, deletion of <i>frh</i> is lethal for the viability of <i>N. crassa</i>, making it elusive whether FRH is essential or nonessential for the circadian rhythm. This needs clarification in a fungus where <i>frh</i> deletion is not lethal. Here, the nuclear FRH ortholog proved nonessential for the circadian rhythm of <i>Metarhizium</i><i>robertsii</i>. The nucleocytoplasmic shuttling of <i>M. robertsii</i> FRQ, WC1, and WC2 orthologs was light-dependent. Yeast two-hybrid assay validated interactions of FRQ with FRH and WC1 instead of FRH with WC1 and WC2 or FRQ with WC2. The circadian rhythm wel"],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm of <i>Metarhizium</i><i>robertsii</i>."],"pmcid":["PMC9224670"],"funding_grant_id":["2022C02058","31772218"],"pubmed_authors":["Peng H","Zhang YL","Ying SH","Feng MG"],"additional_accession":[]},"is_claimable":false,"name":"The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm of <i>Metarhizium</i><i>robertsii</i>.","description":"FRQ (frequency protein), FRH (FRQ-interacting RNA helicase), and WC1 and WC2 (white collar proteins) are major clock elements that govern the circadian rhythm in <i>Neurospora</i><i>crassa</i>. However, deletion of <i>frh</i> is lethal for the viability of <i>N. crassa</i>, making it elusive whether FRH is essential or nonessential for the circadian rhythm. This needs clarification in a fungus where <i>frh</i> deletion is not lethal. Here, the nuclear FRH ortholog proved nonessential for the circadian rhythm of <i>Metarhizium</i><i>robertsii</i>. The nucleocytoplasmic shuttling of <i>M. robertsii</i> FRQ, WC1, and WC2 orthologs was light-dependent. Yeast two-hybrid assay validated interactions of FRQ with FRH and WC1 instead of FRH with WC1 and WC2 or FRQ with WC2. The circadian rhythm wel","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-08-31T03:17:25.079Z","creation":"2025-02-19T02:15:37.484Z"},"accession":"S-EPMC9224670","cross_references":{"pubmed":["35736041"],"doi":["10.3390/jof8060558"]}}