{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Thiebaut AM"],"funding":["Swiss National Science Foundation"],"pagination":["1297-1317"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9225202"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(6)"],"pubmed_abstract":["Cerebral ischemia is a pathology involving a cascade of cellular mechanisms, leading to the deregulation of proteostasis, including macroautophagy/autophagy, and finally to neuronal death. If it is now accepted that cerebral ischemia induces autophagy, the effect of thrombolysis/energy recovery on proteostasis remains unknown. Here, we investigated the effect of thrombolysis by PLAT/tPA (plasminogen activator, tissue) on autophagy and neuronal death. In two <i>in vitro</i> models of hypoxia reperfusion and an <i>in vivo</i> model of thromboembolic stroke with thrombolysis by PLAT/tPA, we found that ischemia enhances neuronal deleterious autophagy. Interestingly, PLAT/tPA decreases autophagy to mediate neuroprotection by modulating the PI3K-AKT-MTOR pathways both <i>in vitro</i> and <i>in v"],"journal":["Autophagy"],"pubmed_title":["Thrombolysis by PLAT/tPA increases serum free IGF1 leading to a decrease of deleterious autophagy following brain ischemia."],"pmcid":["PMC9225202"],"funding_grant_id":["310030","182332","163064"],"pubmed_authors":["Buendia I","Lebouvier L","Roussel BD","Oury F","Thiebaut AM","Lemarchand E","Maillasson M","Rubio M","Boudjadja MB","Ginet V","Vivien D","Hommet Y","Montaner J","Lechevallier C","Puyal J","Hedou E","Deglon N"],"additional_accession":[]},"is_claimable":false,"name":"Thrombolysis by PLAT/tPA increases serum free IGF1 leading to a decrease of deleterious autophagy following brain ischemia.","description":"Cerebral ischemia is a pathology involving a cascade of cellular mechanisms, leading to the deregulation of proteostasis, including macroautophagy/autophagy, and finally to neuronal death. If it is now accepted that cerebral ischemia induces autophagy, the effect of thrombolysis/energy recovery on proteostasis remains unknown. Here, we investigated the effect of thrombolysis by PLAT/tPA (plasminogen activator, tissue) on autophagy and neuronal death. In two <i>in vitro</i> models of hypoxia reperfusion and an <i>in vivo</i> model of thromboembolic stroke with thrombolysis by PLAT/tPA, we found that ischemia enhances neuronal deleterious autophagy. Interestingly, PLAT/tPA decreases autophagy to mediate neuroprotection by modulating the PI3K-AKT-MTOR pathways both <i>in vitro</i> and <i>in v","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-09T16:18:22.137Z","creation":"2024-11-21T01:23:34.104Z"},"accession":"S-EPMC9225202","cross_references":{"pubmed":["34520334"],"doi":["10.1080/15548627.2021.1973339"]}}