biostudies-literatureZheng HUniversity Grants Committee1223https://www.ebi.ac.uk/biostudies/studies/S-EPMC9227628biostudies-literatureUnknown14(6)The multi-inlet vortex mixer (MIVM) has emerged as a novel bottom-up technology for solid nanoparticle preparation. However, its performance in liposome preparation remains unknown. Here, two key process parameters (aqueous/organic flow rate ratio (FRR) and total flow rate (TFR)) of MIVM were investigated for liposome preparation. For this study, two model drugs (lysozyme and erythromycin) were chosen for liposome encapsulation as the representative hydrophilic and hydrophobic drugs, respectively. In addition, two modified MIVMs, one with herringbone-patterned straight inlets and one with zigzag inlets, were designed to further improve the mixing efficiency, aiming to achieve better drug encapsulation. Data showed that FRR played an important role in liposome size control, and a size of &lPharmaceuticsPreparation of Drug-Loaded Liposomes with Multi-Inlet Vortex Mixers.PMC922762824300619Zheng ZZheng HTao HWan JLee KYLeung SSYfalsePreparation of Drug-Loaded Liposomes with Multi-Inlet Vortex Mixers.The multi-inlet vortex mixer (MIVM) has emerged as a novel bottom-up technology for solid nanoparticle preparation. However, its performance in liposome preparation remains unknown. Here, two key process parameters (aqueous/organic flow rate ratio (FRR) and total flow rate (TFR)) of MIVM were investigated for liposome preparation. For this study, two model drugs (lysozyme and erythromycin) were chosen for liposome encapsulation as the representative hydrophilic and hydrophobic drugs, respectively. In addition, two modified MIVMs, one with herringbone-patterned straight inlets and one with zigzag inlets, were designed to further improve the mixing efficiency, aiming to achieve better drug encapsulation. Data showed that FRR played an important role in liposome size control, and a size of &l2022-01-01T00:00:00Z2022 Jun2025-04-04T19:06:29.325Z2025-04-04T19:06:29.325ZS-EPMC92276283574579610.3390/pharmaceutics14061223