biostudies-literatureHanafy ZUnited States Department of AgricultureUSDA National Institute of Food and AgricultureUnited States Department of Agriculture, Agricultural Research Service1194https://www.ebi.ac.uk/biostudies/studies/S-EPMC9227638biostudies-literatureUnknown10(6)<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. <i>C. jejuni</i> and <i>C. coli</i> are naturally competent for transformation via naked DNA uptake, but potential differences in transformation frequency (TF) for different antimicrobial resistance (AMR) markers remain poorly understood. We determined TFs for resistance to different antibiotics using as recipient a derivative of <i>C. jejuni</i> NCTC 11168 (strain SN:CM) with donor DNA from multidrug-resistant <i>C. jejuni</i> or <i>C. coli</i>. TF for nalidixic acid resistance ranked significantly highest (~1.4 × 10^-3), followed by resistance to streptomycin and gentamiMicroorganismsDifferences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i>.PMC92276382030-42000-055-00D2018-67017-27927Olson JWHanafy ZKathariou SMiller WGJackson JHOsborne JAParker CTfalseDifferences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i>.<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. <i>C. jejuni</i> and <i>C. coli</i> are naturally competent for transformation via naked DNA uptake, but potential differences in transformation frequency (TF) for different antimicrobial resistance (AMR) markers remain poorly understood. We determined TFs for resistance to different antibiotics using as recipient a derivative of <i>C. jejuni</i> NCTC 11168 (strain SN:CM) with donor DNA from multidrug-resistant <i>C. jejuni</i> or <i>C. coli</i>. TF for nalidixic acid resistance ranked significantly highest (~1.4 × 10^-3), followed by resistance to streptomycin and gentami2022-01-01T00:00:00Z2022 Jun2026-05-07T03:13:48.146Z2024-11-15T12:45:37.275ZS-EPMC92276383574471210.3390/microorganisms10061194