biostudies-literatureMa TModern Agro-industry Technology Research SystemChina National Tobacco CorporationNational Natural Science Foundation of China1324https://www.ebi.ac.uk/biostudies/studies/S-EPMC9227848biostudies-literatureUnknown14(6)Remorin (REM) is a plant-specific plasma membrane-associated protein regulating plasmodesmata plasticity and restricting viral cell-to-cell movement. Here, we show that palmitoylation is broadly present in group 1 remorin proteins in <i>Nicotiana benthamiana</i> and is crucial for plasma membrane localization and accumulation. By screening the four members of <i>N. benthamiana</i> group 1 remorin proteins, we found that only NbREM1.5 could significantly hamper tobacco mosaic virus (TMV) cell-to-cell movement. We further showed that NbREM1.5 interacts with the movement protein of TMV in vivo and interferes with its function of expanding the plasmodesmata size exclusion limit. We also demonstrated that palmitoylation is indispensable for NbREM1.5 to hamper plasmodesmata permeability and inhiVirusesPalmitoylation Is Indispensable for Remorin to Restrict Tobacco Mosaic Virus Cell-to-Cell Movement in <i>Nicotiana benthamiana</i>.PMC9227848nycytx-001110202001030(JY-13)31930092Wu JFu SMa TWang YZhou XWang KfalsePalmitoylation Is Indispensable for Remorin to Restrict Tobacco Mosaic Virus Cell-to-Cell Movement in <i>Nicotiana benthamiana</i>.Remorin (REM) is a plant-specific plasma membrane-associated protein regulating plasmodesmata plasticity and restricting viral cell-to-cell movement. Here, we show that palmitoylation is broadly present in group 1 remorin proteins in <i>Nicotiana benthamiana</i> and is crucial for plasma membrane localization and accumulation. By screening the four members of <i>N. benthamiana</i> group 1 remorin proteins, we found that only NbREM1.5 could significantly hamper tobacco mosaic virus (TMV) cell-to-cell movement. We further showed that NbREM1.5 interacts with the movement protein of TMV in vivo and interferes with its function of expanding the plasmodesmata size exclusion limit. We also demonstrated that palmitoylation is indispensable for NbREM1.5 to hamper plasmodesmata permeability and inhi2022-01-01T00:00:00Z2022 Jun2026-04-08T10:26:33.41Z2025-02-19T02:57:20.984ZS-EPMC92278483574679510.3390/v14061324