{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sheshukova EV"],"funding":["Russian Foundation for Basic Research","Russian Science Foundation"],"pagination":["1530"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9229031"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(12)"],"pubmed_abstract":["Plants are a promising platform for recombinant protein production. Here we propose a novel approach to increase the level of viral vector-mediated recombinant protein synthesis. This approach is based on the hypothesis that antiviral protection is weakened during the antibacterial cellular response. We suggested that introduced to the cell foreign nuclear localized proteins, including effectors such as bacterial nucleomodulins, can interfere with the import of cellular nuclear proteins and launch antibacterial defense reactions, creating favorable conditions for cytoplasmic virus reproduction. Here, we performed synthesis of an artificial nuclear protein-red fluorescent protein (mRFP) fused with a nuclear localization sequence (NLS)-in plant cells as a mimetic of a bacterial effector. Sup"],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of <i>γ-Thionin</i> Expression."],"pmcid":["PMC9229031"],"funding_grant_id":["19-74-20031","18-34-00576"],"pubmed_authors":["Sheshukova EV","Lipskerov FA","Komarova TV","Ershova NM"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of <i>γ-Thionin</i> Expression.","description":"Plants are a promising platform for recombinant protein production. Here we propose a novel approach to increase the level of viral vector-mediated recombinant protein synthesis. This approach is based on the hypothesis that antiviral protection is weakened during the antibacterial cellular response. We suggested that introduced to the cell foreign nuclear localized proteins, including effectors such as bacterial nucleomodulins, can interfere with the import of cellular nuclear proteins and launch antibacterial defense reactions, creating favorable conditions for cytoplasmic virus reproduction. Here, we performed synthesis of an artificial nuclear protein-red fluorescent protein (mRFP) fused with a nuclear localization sequence (NLS)-in plant cells as a mimetic of a bacterial effector. Sup","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-18T13:50:09.362Z","creation":"2025-04-06T23:36:22.115Z"},"accession":"S-EPMC9229031","cross_references":{"pubmed":["35736681"],"doi":["10.3390/plants11121530"]}}