biostudies-literatureShi JNational Natural Science Foundation of ChinaPriority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)National Key Research and Development Program of China367https://www.ebi.ac.uk/biostudies/studies/S-EPMC9229947biostudies-literatureUnknown14(6)Beauvericin (BEA), a food-borne mycotoxin metabolite derived from the fungus <i>Beauveria Bassiana</i>, is proven to exhibit high hepatotoxicity. However, the molecular mechanism underlying BEA-induced liver damage is not fully understood. Herein, the effect of Nrf2 nuclear translocation-induced by BEA in hepatocytes was investigated. CCK8 solution was used to determine the appropriate concentrations of BEA (0, 1, 1.5 and 2 μmol/L), and BRL3A cells were then exposed to different concentrations of BEA for 12 h. Our results reveal that BEA exposure is associated with high cytotoxicity, lowered cell viability, damaged cellular morphology, and increased apoptosis rate. BEA could lead to oxidative damage through the overproduction of ROS and unbalanced redox, trigger the activation of Nrf2 signToxinsRole of Nrf2 Nucleus Translocation in Beauvericin-Induced Cell Damage in Rat Hepatocytes.PMC9229947321729212016YFD0501208Zou HLiu ZGu JBian JShi JWang YXu WCai GYuan YfalseRole of Nrf2 Nucleus Translocation in Beauvericin-Induced Cell Damage in Rat Hepatocytes.Beauvericin (BEA), a food-borne mycotoxin metabolite derived from the fungus <i>Beauveria Bassiana</i>, is proven to exhibit high hepatotoxicity. However, the molecular mechanism underlying BEA-induced liver damage is not fully understood. Herein, the effect of Nrf2 nuclear translocation-induced by BEA in hepatocytes was investigated. CCK8 solution was used to determine the appropriate concentrations of BEA (0, 1, 1.5 and 2 μmol/L), and BRL3A cells were then exposed to different concentrations of BEA for 12 h. Our results reveal that BEA exposure is associated with high cytotoxicity, lowered cell viability, damaged cellular morphology, and increased apoptosis rate. BEA could lead to oxidative damage through the overproduction of ROS and unbalanced redox, trigger the activation of Nrf2 sign2022-01-01T00:00:00Z2022 May2025-08-12T03:04:40.357Z2025-04-05T12:38:59.198ZS-EPMC92299473573702810.3390/toxins14060367