{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cheng Y"],"funding":["Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles","NIAID NIH HHS","NINDS NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["110885"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9230077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(9)"],"pubmed_abstract":["Physiological blood-tissue barriers play a critical role in separating the circulation from immune-privileged sites and denying access to blood-borne viruses. The mechanism of virus restriction by these barriers is poorly understood. We utilize induced pluripotent stem cell (iPSC)-derived human brain microvascular endothelial cells (iBMECs) to study virus-blood-brain barrier (BBB) interactions. These iPSC-derived cells faithfully recapitulate a striking difference in in vivo neuroinvasion by two alphavirus isolates and are selectively permissive to neurotropic flaviviruses. A model of cocultured iBMECs and astrocytes exhibits high transendothelial electrical resistance and blocks non-neurotropic flaviviruses from getting across the barrier. We find that iBMECs constitutively express an int"],"journal":["Cell reports"],"pubmed_title":["Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model."],"pmcid":["PMC9230077"],"funding_grant_id":["R35 NS097370","R35 NS116843","R21OD024896","R35NS116843","R21 OD024896","R35NS097370","U19AI131130","R01 AI158704","R01AI158704","R01 AI146342","U19 AI131130","R01AI146342"],"pubmed_authors":["Natekar JP","Basu M","Lang J","Li MMH","Wen Z","Brinton MA","Nkembo MB","Xu C","Kumar M","Song H","Nguyen PTT","Cheng Y","Tang H","Qian X","Yao Z","Sanchez E","Ming GL","Medina A"],"additional_accession":[]},"is_claimable":false,"name":"Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.","description":"Physiological blood-tissue barriers play a critical role in separating the circulation from immune-privileged sites and denying access to blood-borne viruses. The mechanism of virus restriction by these barriers is poorly understood. We utilize induced pluripotent stem cell (iPSC)-derived human brain microvascular endothelial cells (iBMECs) to study virus-blood-brain barrier (BBB) interactions. These iPSC-derived cells faithfully recapitulate a striking difference in in vivo neuroinvasion by two alphavirus isolates and are selectively permissive to neurotropic flaviviruses. A model of cocultured iBMECs and astrocytes exhibits high transendothelial electrical resistance and blocks non-neurotropic flaviviruses from getting across the barrier. We find that iBMECs constitutively express an int","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2026-05-10T01:40:33.608Z","creation":"2025-02-19T02:57:19.544Z"},"accession":"S-EPMC9230077","cross_references":{"pubmed":["35649379"],"doi":["10.1016/j.celrep.2022.110885"]}}