biostudies-literatureKaundal RDepartment of Science &amp; Technology3867https://www.ebi.ac.uk/biostudies/studies/S-EPMC9230710biostudies-literatureUnknown27(12)<h4>Background</h4>Himalayan Viola species (Banksha) are traditionally important herbs with versatile therapeutic benefits such as antitussive, analgesic, antipyretic, antimalarial, anti-inflammatory, and anticancerous ones. The current investigation was focused on exploring polyphenolic profiles, antioxidant, and antimicrobial potentials of wild viola species at 15 gradient locations (375-1829 m).<h4>Methods</h4>Morphological, physiochemical, and proximate analyses were carried out as per WHO guidelines for plant drug standardization. Total polyphenolic and flavonoid content were carried out using gallic acid and rutin equivalent. UPLC-DAD was used to profile the targeted polyphenols (gallic acid, vanillic acid, syringic acid, <i>p</i>-coumaric acid, ferulic acid, rutin, quercetin, luteolMolecules (Basel, Switzerland)Polyphenolic Profiling, Antioxidant, and Antimicrobial Activities Revealed the Quality and Adaptive Behavior of Viola Species, a Dietary Spice in the Himalayas.PMC9230710DST-INSPIRE (No. DST/INSPIRE Fellowship/2018/IF180988)Kumar DKaundal RKumar MSingh DKumar SfalsePolyphenolic Profiling, Antioxidant, and Antimicrobial Activities Revealed the Quality and Adaptive Behavior of Viola Species, a Dietary Spice in the Himalayas.<h4>Background</h4>Himalayan Viola species (Banksha) are traditionally important herbs with versatile therapeutic benefits such as antitussive, analgesic, antipyretic, antimalarial, anti-inflammatory, and anticancerous ones. The current investigation was focused on exploring polyphenolic profiles, antioxidant, and antimicrobial potentials of wild viola species at 15 gradient locations (375-1829 m).<h4>Methods</h4>Morphological, physiochemical, and proximate analyses were carried out as per WHO guidelines for plant drug standardization. Total polyphenolic and flavonoid content were carried out using gallic acid and rutin equivalent. UPLC-DAD was used to profile the targeted polyphenols (gallic acid, vanillic acid, syringic acid, <i>p</i>-coumaric acid, ferulic acid, rutin, quercetin, luteol2022-01-01T00:00:00Z2022 Jun2025-04-04T19:13:12.752Z2025-04-04T19:13:12.752ZS-EPMC92307103574498910.3390/molecules27123867