biostudies-literatureZhang YNational Institute of HealthNational Institutes of HealthNational Institute of General Medical SciencesNIGMS NIH HHS22-28https://www.ebi.ac.uk/biostudies/studies/S-EPMC9233151biostudies-literatureUnknown204A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.Methods (San Diego, Calif.)Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.PMC9233151R15 GM1147512R15GM114751-02Olowolagba ALiu HArachchige DLZhang YLuck RLfalseNear-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.2022-01-01T00:00:00Z2022 Aug2025-04-04T20:07:43.31Z2025-04-04T20:07:43.31ZS-EPMC92331513538133710.1016/j.ymeth.2022.03.019