biostudies-literatureUnknown13Qin YFlavonoids were the major phytochemicals against hepatic peroxidative injury in <i>Scleromitron diffusum</i> (Willd.) R. J. Wang with an inventive bio-enzymatic method by our group (LU500041). Firstly, the total flavonoids from <i>Scleromitron diffusum</i> (Willd.) R. J. Wang were extracted by reflux, ultrasonic, ultrasound-assisted enzymatic methods (TFH), and the bio-enzymatic method (Ey-TFH). Then 24 flavonoid compounds were isolated and quantified in the extracts by UPLC-MS/MS. Next, six representative differential compounds in Ey-TFH were further screened out by multivariate statistical analysis compared with those in TFH. In a further step, Ey-TFH presented a higher protective rate (59.30 ± 0.81%) against H₂O₂-damaged HL-02 hepatocytes than TFH. And six represenFrontiers in pharmacology890148https://www.ebi.ac.uk/biostudies/studies/S-EPMC9234865biostudies-literatureIsolation and Quantification of the Hepatoprotective Flavonoids From <i>Scleromitron diffusum</i> (Willd.) R. J. Wang With Bio-Enzymatic Method Against NAFLD by UPLC-MS/MS.PMC9234865Qiao YLi YZhao BShi CQin YLiu QZhang MDeng HfalseIsolation and Quantification of the Hepatoprotective Flavonoids From <i>Scleromitron diffusum</i> (Willd.) R. J. Wang With Bio-Enzymatic Method Against NAFLD by UPLC-MS/MS.Flavonoids were the major phytochemicals against hepatic peroxidative injury in <i>Scleromitron diffusum</i> (Willd.) R. J. Wang with an inventive bio-enzymatic method by our group (LU500041). Firstly, the total flavonoids from <i>Scleromitron diffusum</i> (Willd.) R. J. Wang were extracted by reflux, ultrasonic, ultrasound-assisted enzymatic methods (TFH), and the bio-enzymatic method (Ey-TFH). Then 24 flavonoid compounds were isolated and quantified in the extracts by UPLC-MS/MS. Next, six representative differential compounds in Ey-TFH were further screened out by multivariate statistical analysis compared with those in TFH. In a further step, Ey-TFH presented a higher protective rate (59.30 ± 0.81%) against H₂O₂-damaged HL-02 hepatocytes than TFH. And six represen2022-01-01T00:00:00Z20222025-04-04T11:28:31.209Z2025-04-04T11:28:31.209ZS-EPMC92348653577008010.3389/fphar.2022.890148