biostudies-literatureUnknown2022Luo Y<h4>Purpose</h4>We previously reported that G protein-coupled receptor kinase (GRK) 4 halts cell cycle progression and induces cellular senescence in HEK293 cells. The present study was aimed at assessing the prognostic value of GRK4 in hepatocellular carcinoma (HCC).<h4>Methods</h4>GRK4 expression was detected by immunohistochemistry in paired tumoral and peritumoral tissues of 325 HCC patients. One hundred and twenty-six patients from Western China were utilized as a training cohort to develop a nomogram, while 86 patients from Eastern China were used as a validation cohort. The proliferation and migration of lentiviral-GRK4 expressing HepG2 cells were determined by MTT and wound healing assays.<h4>Results</h4>GRK4 was differentially expressed in HCC tissues. Tumoral GRK4 intensity, tumoDisease markers2628879https://www.ebi.ac.uk/biostudies/studies/S-EPMC9236775biostudies-literatureG Protein-Coupled Receptor Kinase 4 Is a Novel Prognostic Factor in Hepatocellular Carcinoma.PMC9236775Jiang XLuo YLi RWang ZXiao SfalseG Protein-Coupled Receptor Kinase 4 Is a Novel Prognostic Factor in Hepatocellular Carcinoma.<h4>Purpose</h4>We previously reported that G protein-coupled receptor kinase (GRK) 4 halts cell cycle progression and induces cellular senescence in HEK293 cells. The present study was aimed at assessing the prognostic value of GRK4 in hepatocellular carcinoma (HCC).<h4>Methods</h4>GRK4 expression was detected by immunohistochemistry in paired tumoral and peritumoral tissues of 325 HCC patients. One hundred and twenty-six patients from Western China were utilized as a training cohort to develop a nomogram, while 86 patients from Eastern China were used as a validation cohort. The proliferation and migration of lentiviral-GRK4 expressing HepG2 cells were determined by MTT and wound healing assays.<h4>Results</h4>GRK4 was differentially expressed in HCC tissues. Tumoral GRK4 intensity, tumo2022-01-01T00:00:00Z20222025-04-21T14:20:52.408Z2025-04-21T14:20:52.408ZS-EPMC92367753576981610.1155/2022/2628879