biostudies-literatureCai YMShenzhen Science and technology programGuangdong Natural Science Foundation for Distinguished Young ScholarShenzhen Key laboratory of gene regulation and systems biologye0003922https://www.ebi.ac.uk/biostudies/studies/S-EPMC9238385biostudies-literatureUnknown88(12)In Pseudomonas aeruginosa PAO1, 41 genes encode proteins predicted to be involved in the production or degradation of c-di-GMP, a ubiquitous secondary messenger that regulates a variety of physiological behaviors closely related to biofilm and aggregate formation. Despite extensive effort, the entire picture of this important signaling network is still unclear, with one-third of these proteins remaining uncharacterized. Here, we show that the deletion of <i>pipA</i>, which produces a protein containing two PAS domains upstream of a GGDEF-EAL tandem, significantly increased the intracellular c-di-GMP level and promoted the formation of aggregates both on surfaces and in planktonic cultures. However, this regulatory effect was not contributed by either of the two classic pathways modulating Applied and environmental microbiologyThe c-di-GMP Phosphodiesterase PipA (PA0285) Regulates Autoaggregation and Pf4 Bacteriophage Production in Pseudomonas aeruginosa PAO1.PMC9238385KQTD202009091137580042020B1515020003ZDSYS20200811144002008Zhou ZHLiu YCai YMYang LCai ZYu KWLiu JHWang TFfalseThe c-di-GMP Phosphodiesterase PipA (PA0285) Regulates Autoaggregation and Pf4 Bacteriophage Production in Pseudomonas aeruginosa PAO1.In Pseudomonas aeruginosa PAO1, 41 genes encode proteins predicted to be involved in the production or degradation of c-di-GMP, a ubiquitous secondary messenger that regulates a variety of physiological behaviors closely related to biofilm and aggregate formation. Despite extensive effort, the entire picture of this important signaling network is still unclear, with one-third of these proteins remaining uncharacterized. Here, we show that the deletion of <i>pipA</i>, which produces a protein containing two PAS domains upstream of a GGDEF-EAL tandem, significantly increased the intracellular c-di-GMP level and promoted the formation of aggregates both on surfaces and in planktonic cultures. However, this regulatory effect was not contributed by either of the two classic pathways modulating 2022-01-01T00:00:00Z2022 Jun2026-06-13T06:06:49.629Z2025-04-19T15:41:02.006ZS-EPMC92383853563884510.1128/aem.00039-22