biostudies-literatureLi JAmerican Cancer SocietyNHLBI NIH HHSNCI NIH HHSWelch FoundationNational Institutes of HealthCancer Prevention and Research Institute of TexasNIGMS NIH HHS81https://www.ebi.ac.uk/biostudies/studies/S-EPMC9241241biostudies-literatureUnknown14(1)Epigenetic abnormalities in DNA hydroxymethylation (5hmC) have been detected in patients with myeloid neoplasms, suggesting that 5hmC might act as a valuable epigenetic mark to reflect the disease status of myeloid neoplasms. Here, we report systematic genome-wide mapping of the DNA hydroxymethylomes in over 70 patients with myeloid neoplasms. Our integrative analysis leads to the identification of distinct 5hmC signatures that can sensitively discriminate patients from healthy individuals. At the molecular level, we unveiled dynamic 5hmC changes within key transcription factor (e.g., the CEBP family) binding motifs that are essential for hematopoiesis and myeloid lineage specification. 5hmC redistribution was found to alter the genome-wide binding of CEBP-α, thereby reprogramming transcriClinical epigeneticsAberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasms.PMC9241241CA016672R01HL134780RSG-18-043-01-LIBR01CA232017R21 GM138824R01 CA232017R01 HL146852BE-1913-20190330RP210070RP180131R01 HL134780R01 CA240258P01 CA265748Yang HClass CLi JLee MHe HSiwko SZhou YWei YYou MJGarcia-Manero GGuo LYang YHuang YHong TWang XfalseAberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasms.Epigenetic abnormalities in DNA hydroxymethylation (5hmC) have been detected in patients with myeloid neoplasms, suggesting that 5hmC might act as a valuable epigenetic mark to reflect the disease status of myeloid neoplasms. Here, we report systematic genome-wide mapping of the DNA hydroxymethylomes in over 70 patients with myeloid neoplasms. Our integrative analysis leads to the identification of distinct 5hmC signatures that can sensitively discriminate patients from healthy individuals. At the molecular level, we unveiled dynamic 5hmC changes within key transcription factor (e.g., the CEBP family) binding motifs that are essential for hematopoiesis and myeloid lineage specification. 5hmC redistribution was found to alter the genome-wide binding of CEBP-α, thereby reprogramming transcri2022-01-01T00:00:00Z2022 Jun2026-07-14T16:20:42.55Z2022-07-08T10:35:01.081ZS-EPMC92412413576505210.1186/s13148-022-01297-5